› 1xxn

X-ray diffraction
1.7Å resolution

Crystal structure of a mesophilic xylanase A from Bacillus subtilis 1A1

Released:
DOI: 10.2210/pdb1xxn/pdb
PDB entry 1xxn coloured by chain, front view.
PDB entry 1xxn coloured by chain, side view.
PDB entry 1xxn coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
Correlation of temperature induced conformation change with optimum catalytic activity in the recombinant G/11 xylanase A from Bacillus subtilis strain 168 (1A1).
Murakami MT, Arni RK, Vieira DS, Degrève L, Ruller R, Ward RJ
FEBS Lett 579 6505-10 (2005)
PMID: 16289057

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148365 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endo-1,4-beta-xylanase A Chain: A
Molecule details ›
Chain: A
Length: 185 amino acids
Theoretical weight: 20.39 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P18429 (Residues: 29-213; Coverage: 100%)
Gene names: BSU18840, xynA
Sequence domains: Glycosyl hydrolases family 11
Structure domains: Jelly Rolls

Ligands and Environments

1 bound ligand:
Ligand SRT 1 x SRT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: LNLS BEAMLINE D03B-MX1
Spacegroup: P21212
Unit cell:
a: 50.931Å b: 70.499Å c: 42.044Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.173 0.171 0.212
Expression system: Escherichia coli BL21

Quick links

Citations

2 review citations

Structural determinants of the substrate specificities of xylanases from different glycoside hydrolase families.
Pollet et al. (2010)
1 more

13 mentions without citation

Engineering the pattern of protein glycosylation modulates the thermostability of a GH11 xylanase.
Fonseca-Maldonado et al. (2013)
12 more