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"secondary-accession": "ERP009566",
"centre-name": "ALFRED WEGENER INSTITUTE FOR POLAR AND MARINE RESE",
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"study-abstract": "We transplanted oysters from two invasive, genetically distinct invasive populations of the Wadden sea (Texel, Netherlands and Sylt, Germany, and monitored the short term microbiota shifts and colonization in different tissues during the initial phases of establishment in the new habitat. To manipulate the microbiome mismatch we treated half of the oyster with antibiotics in order to minimize the interactions between resident microbiota and new colonizers, while the other half was transplanted with their natural resident microbiome. We followed oyster survival and changes in diversity, composition and abundance of oyster-associated bacterial communities as a whole and Vibrionaceae and Arcobacter in particular, over the first five days in the new habitat. In this way, we could estimate how the composition and diversity of microbiota in different tissues contribute to success of oyster establishment in the new environment.",
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"accession": "MGYS00001607",
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"last-update": "2017-03-21T11:42:48",
"secondary-accession": "ERP010751",
"centre-name": "ALFRED WEGENER INSTITUTE FOR POLAR AND MARINE RESE",
"public-release-date": null,
"study-abstract": "Pacific oysters (Crassostrea gigas) from two genetically differentiated populations from the Wadden Sea - one from Texel, the other from Sylt - were reciprocally translocated between the sites at the beginning of June 2012. Half of the oysters were additionally pretreated with antibiotics to remove resident microbiota. Local controls were included as well, resulting in four groups at each site: antibiotic treated local or translocated, and control local or translocated oysters.Hemolymph samples were taken immediately after the pretreatment in the lab and then on 3 (monthly, Texel) and 5 (biweekly, Sylt) occasions in the field. Exact position of each oyster in the field was recorded, so that the data allow for study of spatial patterns of oyster hemolymph microbiota. One seawater sample is included for reference for each sampling point.",
"study-name": "Natural variation of Pacific oyster hemolymph microbiota at two sites in the Wadden Sea (Texel, Netherlands, & Sylt, Germany) throughout the summer season 2012",
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{
"type": "studies",
"id": "MGYS00001491",
"attributes": {
"bioproject": "PRJEB9174",
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"accession": "MGYS00001491",
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"last-update": "2017-02-15T09:56:55",
"secondary-accession": "ERP010257",
"centre-name": "SOUTHERNCROSSPLANTSCIENCE",
"public-release-date": null,
"study-abstract": "Dicathais orbita is a marine mollusc well known for the production of anticancer compounds that are precursors to Tyrian purple. This study aimed to assess the full diversity of bacteria associated with the Tyrian purple producing hypobranchial gland and to identify bacteria specifically associated with the purple producing gland by comparing with the foot tissues using a high-throughput sequencing approach. The total genomic DNA was extracted from the hypobranchial gland and foot of D. orbita for high-throughput sequencing targeting the variable region V1-V3 of 16S rRNA bacterial gene. 16S rRNA bacterial sequences were processed by using combinations of QIIME and MEGAN software. The metagenome analysis revealed highly diverse bacterial assemblage associated with the hypobranchial gland and foot tissues of D. orbita. The most dominant bacteria phylum in the metagenome data set was Proteobacteria followed by Tenericutes, Spirochaetes and Bacteroidetes. Our result has also provided that larger numbers of bacterial taxa were present in the foot than the hypobranchial gland of D. orbita. This study also revealed the high abundance of Vibrio spp. in the Tyrian purple producing gland of a muricid mollusc. It also reports the presence of unique bacterial communities which shows indole production, bromoperoxidase and brominated secondary metabolites in the biosynthetic gland. This study reveals the full diversity of bacteria associated with the Tyrian purple producing gland of a marine muricids and also helped in establishing the potential role of bacteria in the biosynthesis of Tyrian purple.",
"study-name": "Metagenomic profiling of bacterial communities associated with Tyrian Purple production in a marine gastropod",
"data-origination": "SUBMITTED"
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