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"study-abstract": "Microbial activity in lake sediments plays a key role in the cycling of nutrients and organic matter that continuously sink from water columns. As organic matter accumulates at the sediment surface, upper sediment layers become buried, resulting in a decrease in microbial activity and cell turnover rate with increasing depth. To better understand the structure of freshwater sediment communities and how the burial processes influence it, we quantified the distribution of a broad range of environmental parameters and the community structure of microbial archaea, bacteria, and eukaryotes in the sediments of a clear, temperate lake. Our findings suggest that the two uppermost horizons of the sediment community, which cover approximately the last 70 years, are characterized by a high taxa replacement influenced by “present” environmental parameters. Richness effects became increasingly important in the lowest studied horizon (14-30 cm, age 70-150 a) and could be readily explained by conservative “past” environmental parameters. The lowest horizon is also characterized by a switch in dominance toward archaea, as has been found in marine sediments. Our pioneering study shows that the freshwater lake's community structure, taxa, and vertical arrangement share many features previously observed for marine sediments.",
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"study-abstract": "18S rDNA amplicon study of bulk water and shower hose biofilm samples. Cold water samples were collected from the water outlet of a drinking water treatment plant and from the water inlet of two neighboring buildings, warm water samples (ca 37 degrees C) were taken from showers within the two buildings: one building with a copper-silver-ionization-system to disinfect the water, the other building without such a system. Biofilms were taken from shower hoses within the two buildings. For further experimental details see: Stüken et al. 2018. Environ Sci Technol.;52(6):3354-3364. doi: 10.1021/acs.est.7b0596318S rDNA amplicons were generated with forward primer 1391F (GTA CAC ACC GCC CGTC) and reverse primer 1510R (CCT TCY GCA GGT TCA CCT AC). AdapterRemoval ver 2.3.0 and cutadapt vers. 1.18. were used to prepare samples for sequence submission (removal of remaining Illumina adapters, demultiplexing, removal of heterogeneity spacers, primers, low quality bases and sequences < 50 bp lengths).",
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"study-abstract": "The crust of lateritic (canga) is a particular environment to be a ferruginous complex that inhabiting a high species diversity, high endemism and unique species compositions generally metal-tolerant (see Jacobi et al. 2007). Lakes are formed in the top of Canga which are maintained by local rainfall patterns. Information about the formation and dynamics of these lakes are scarce, considering that microorganisms play crucial roles in ecosystem functioning, here we aimed to understand the relationship between microbial diversity, functions and environmental parameters that sustain this peculiar amazonian environment. In this study, we compare the taxonomic and functional structure of the microbial communities from sediment in three neighboring lakes on top of the extensive lateritic crust in the southeast Amazon Basin using shotgun metagenome sequencing",
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"secondary-accession": "ERP022245",
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"study-abstract": "Longterm data (up to 9 years) of 8 lakes situated in Northeastern Germany are provided. Samples were taken monthly on average dividing particle-associated (greater than 5.0um) and free-living (less than 5.0um, greater than 0.2um) microbes in the water column and in two lakes in the sediment. The lakes are all located closely to each other but differ in many physicochemical parameters such as pH and conductivity. Thus, similarities as well as dissimilarities of microbial communities are of high interest. One lake (Lake Tiefwaren) was sampled after phosphate restoration.",
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"study-abstract": "During the construction phase of the Koralm railway tunnel several samples from different sites were analysed for the presence of microbes. 16S rDNA amplicon sequencing and subsequent microbial profiling revealed the presence of bacterial consortia typical for the deep continental subsurface dominated by autotrophs, methanotrophs and methylotrophs.\nReference: Koraimann G.; Bischof K. (2022) Characterisation of microbial biofilms from tunnel drainage water. Geomechanics and Tunnelling. 15 (2022), No. 4",
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"last-update": "2022-07-02T20:15:34",
"secondary-accession": "ERP109674",
"centre-name": "EAWAG",
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"study-abstract": "A detailed sampling of the water column in proximity to the oxygen-methane interface of four stratified Swiss lakes was performed. Amplicon sequencing analysis of 16S rRNA and pmoA genes and 16S rRNA and pmoA transcripts was used to investigate the active methane-oxidizing bacteria inhabiting the oxygen-methane resource gradient.",
"study-name": "Niche partitioning of methanotrophs in stratified lakes",
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"last-update": "2022-03-21T21:57:10",
"secondary-accession": "ERP016854",
"centre-name": "UCSDMI",
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"study-abstract": "Bog lakes such as Mary Lake in northern Wisconsin are ideal systems to investigate microbial communities due to easily identified spatial boundaries and well characterized seasonal ecosystem dynamics. Multiple factors are thought to influence the bacterial communities within the lake, and our goal in this study was to analyze the spatial and temporal factors causing the variations between these populations. Molecular techniques and statistical analyses were performed to gain insight into which differences caused the greatest variability. The bacterial communities were analyzed based on conserved 16S ribosomal RNA sequences as well as on short base pair sequence reads obtained from a time series in 2009 in order to determine whether the bacterial community was more variable in space or in time. We found that while the upper and lower thermal layers of the bog were composed of similar microbial communities, these communities had different temporal patterns throughout the time series.",
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