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{
"metadata": {
"accession": "PS51253",
"entry_id": null,
"type": "domain",
"go_terms": null,
"source_database": "profile",
"member_databases": null,
"integrated": "IPR006600",
"hierarchy": null,
"name": {
"name": "CENPB-type HTH domain profile",
"short": "HTH_CENPB"
},
"description": [
{
"text": "<p>The CENPB-type HTH domain is a DNA-binding, helix-turn-helix (HTH) domain of\nabout 70-75 amino acids, present in eukaryotic centromere proteins and\ntransposases. The domain is named after the mammalian major centromere\nautoantigen B or centromere protein B (CENP-B), which is a fundamental\ncentromere component of chromosomes. The N-terminus of CENP-B contains two\nDNA-binding HTH domains, which bind to adjacent major grooves of DNA. The\nN-terminus of CENP-B is formed by a psq-type HTH domain and\nC-terminal to this domain lies the CENPB-type HTH domain. These two HTH\ndomains together bind specifically to a 17-base-pair sequence, the CENP-B box,\nwhich occurs in alpha-satellite DNA in human centromeres [[cite:PUB00035346]].\n\nThe structure of the CENPB-type HTH domain is composed of three alpha-helices. The second and third helices connected via a turn comprise\nthe helix-turn-helix motif. Helix 3 is termed the recognition helix as it\nbinds the DNA major groove, like in other HTHs. In CENP-B this domain\nrecognizes site 3 of the CENP-B box, while the preceding psq-type HTH binds site 1 of the CENP-B box, and a connecting linker loop\nbinds in the minor groove of DNA and recognizes site 2 [[cite:PUB00025705]].\n\nSome proteins known to contain a CENPB-type HTH domain:\n\n - Mammalian centromere protein B (CENP-B), associated with the centromere and\n specifically binding DNA to the CENP-B box.\n - Mammalian jerky protein, involved in epileptic seizures in mice.\n - Mammalian Pogo transposases.\n - Fission yeast ARS-binding protein 1 (abp1) and CENP-B homolog proteins\n (CBHP-1 and 2), which are centromere proteins.\n - Candida albicans protein PDC2.\n - Fungal transposases.\n\nThe profile we developed covers the entire CENPB-type HTH domain, including\nthe N-terminal linker loop.</p>",
"llm": false,
"checked": false,
"updated": false
}
],
"wikipedia": null,
"literature": {
"PUB00035346": {
"PMID": 16183641,
"ISBN": null,
"volume": "280",
"issue": "50",
"year": 2005,
"title": "Human centromere protein B induces translational positioning of nucleosomes on alpha-satellite sequences.",
"URL": null,
"raw_pages": "41609-18",
"medline_journal": "J Biol Chem",
"ISO_journal": "J. Biol. Chem.",
"authors": [
"Tanaka Y",
"Tachiwana H",
"Yoda K",
"Masumoto H",
"Okazaki T",
"Kurumizaka H",
"Yokoyama S."
],
"DOI_URL": "http://dx.doi.org/10.1074/jbc.M509666200"
},
"PUB00025705": {
"PMID": 11726497,
"ISBN": null,
"volume": "20",
"issue": "23",
"year": 2001,
"title": "Crystal structure of the CENP-B protein-DNA complex: the DNA-binding domains of CENP-B induce kinks in the CENP-B box DNA.",
"URL": null,
"raw_pages": "6612-8",
"medline_journal": "EMBO J",
"ISO_journal": "EMBO J.",
"authors": [
"Tanaka Y",
"Nureki O",
"Kurumizaka H",
"Fukai S",
"Kawaguchi S",
"Ikuta M",
"Iwahara J",
"Okazaki T",
"Yokoyama S."
],
"DOI_URL": "http://dx.doi.org/10.1093/emboj/20.23.6612"
}
},
"set_info": null,
"overlaps_with": null,
"counters": {
"subfamilies": 0,
"domain_architectures": 0,
"interactions": 0,
"matches": 30844,
"pathways": 0,
"proteins": 30463,
"proteomes": 2562,
"sets": 0,
"structural_models": {
"alphafold": 25959,
"bfvd": 0
},
"structures": 2,
"taxa": 7471
},
"entry_annotations": {},
"cross_references": {},
"is_llm": false,
"is_reviewed_llm": false,
"is_updated_llm": false,
"representative_structure": null
}
}
