{"metadata":{"accession":"PS51253","entry_id":null,"type":"domain","go_terms":null,"source_database":"profile","member_databases":null,"integrated":"IPR006600","hierarchy":null,"name":{"name":"CENPB-type HTH domain profile","short":"HTH_CENPB"},"description":[{"text":"<p>The CENPB-type HTH domain is a DNA-binding, helix-turn-helix (HTH) domain of\nabout 70-75 amino acids, present in eukaryotic centromere proteins and\ntransposases. The domain is named after the mammalian major centromere\nautoantigen B or centromere protein B (CENP-B), which is a fundamental\ncentromere component of chromosomes. The N-terminus of CENP-B contains two\nDNA-binding HTH domains, which bind to adjacent major grooves of DNA. The\nN-terminus of CENP-B is formed by a psq-type HTH domain and\nC-terminal to this domain lies the CENPB-type HTH domain. These two HTH\ndomains together bind specifically to a 17-base-pair sequence, the CENP-B box,\nwhich occurs in alpha-satellite DNA in human centromeres [[cite:PUB00035346]].\n\nThe structure of the CENPB-type HTH domain is composed of three alpha-helices. The second and third helices connected via a turn comprise\nthe helix-turn-helix motif. Helix 3 is termed the recognition helix as it\nbinds the DNA major groove, like in other HTHs. In CENP-B this domain\nrecognizes site 3 of the CENP-B box, while the preceding psq-type HTH binds site 1 of the CENP-B box, and a connecting linker loop\nbinds in the minor groove of DNA and recognizes site 2 [[cite:PUB00025705]].\n\nSome proteins known to contain a CENPB-type HTH domain:\n\n - Mammalian centromere protein B (CENP-B), associated with the centromere and\n   specifically binding DNA to the CENP-B box.\n - Mammalian jerky protein, involved in epileptic seizures in mice.\n - Mammalian Pogo transposases.\n - Fission  yeast  ARS-binding  protein  1  (abp1) and CENP-B homolog proteins\n   (CBHP-1 and 2), which are centromere proteins.\n - Candida albicans protein PDC2.\n - Fungal transposases.\n\nThe profile we developed covers the entire CENPB-type HTH domain, including\nthe N-terminal linker loop.</p>","llm":false,"checked":false,"updated":false}],"wikipedia":null,"literature":{"PUB00035346":{"PMID":16183641,"ISBN":null,"volume":"280","issue":"50","year":2005,"title":"Human centromere protein B induces translational positioning of nucleosomes on alpha-satellite sequences.","URL":null,"raw_pages":"41609-18","medline_journal":"J Biol Chem","ISO_journal":"J. Biol. Chem.","authors":["Tanaka Y","Tachiwana H","Yoda K","Masumoto H","Okazaki T","Kurumizaka H","Yokoyama S."],"DOI_URL":"http://dx.doi.org/10.1074/jbc.M509666200"},"PUB00025705":{"PMID":11726497,"ISBN":null,"volume":"20","issue":"23","year":2001,"title":"Crystal structure of the CENP-B protein-DNA complex: the DNA-binding domains of CENP-B induce kinks in the CENP-B box DNA.","URL":null,"raw_pages":"6612-8","medline_journal":"EMBO J","ISO_journal":"EMBO J.","authors":["Tanaka Y","Nureki O","Kurumizaka H","Fukai S","Kawaguchi S","Ikuta M","Iwahara J","Okazaki T","Yokoyama S."],"DOI_URL":"http://dx.doi.org/10.1093/emboj/20.23.6612"}},"set_info":null,"overlaps_with":null,"counters":{"subfamilies":0,"domain_architectures":0,"interactions":0,"matches":30844,"pathways":0,"proteins":30463,"proteomes":2562,"sets":0,"structural_models":{"alphafold":25959,"bfvd":0},"structures":2,"taxa":7471},"entry_annotations":{},"cross_references":{},"is_llm":false,"is_reviewed_llm":false,"is_updated_llm":false,"representative_structure":null}}