loyal-squid alpha

3D UNet Mouse Embryo Fixed

V. Bondarenko, A. Wolny

10.5281/zenodo.6383429/7774505

Example image containing both original image data and after applying the model to it.

Model Information

Description: A 3D U-Net trained to predict the nuclei and their boundaries in fixed confocal images of developing mouse embryo.Voxel size: (0.2×0.2×1 µm^3) (XYZ).
Task: 3D nucleus segmentation
Licence: MIT
Tags: 3D, nuclei, mouse embryo, fixed tissue, confocal microscopy, semantic segmentation, torchscript, ilastik, U-Net, plantseg, deepimagej, pytorch

Benchmarking

Study Dataset Precision Recall IoU Dice Analysis parameters
S-BIAD1518 Scale-cleared rat kidney 0.751 0.024 0.023 0.046 z_slices_analysed: 1,11
S-BIAD1492 Simulated fluorescence images 1 0.376 0.376 0.546 z_slices_analysed: 58,68
S-BIAD1518 Human kidney cortex 0.778 0.171 0.163 0.281 z_slices_analysed: 3,13
S-BIAD1518 Human kidney nephrectomy 0.743 0.001 0.001 0.001 z_slices_analysed: 11,21
S-BIAD1518 Cleared mouse intestine 0.775 0.256 0.239 0.385 z_slices_analysed: 14,24
S-BIAD1518 Synthetic cell nuclei 0.973 0.151 0.15 0.261 z_slices_analysed: 58,68
S-BIAD1196 brain cell nucleus patches 0.32 0.155 0.117 0.209 z_slices_analysed: 94,104
S-BIAD1026 Go-Nuclear image dataset 0.999 0.33 0.329 0.496 z_slices_analysed: 126,136
S-BIAD1518 BABB-cleared kidney 1 0.139 0.139 0.244 z_slices_analysed: 58,68
S-BIAD1518 Biopsy of diabetic human kidney 0.927 0.177 0.175 0.298 z_slices_analysed: 11,21

Model predictions

Study Dataset Original image Reference annotation Prediction Analysis parameters
S-BIAD1518 Scale-cleared rat kidney
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z_slices_analysed: 1,11
S-BIAD1492 Simulated fluorescence images
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z_slices_analysed: 58,68
S-BIAD1518 Human kidney cortex
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z_slices_analysed: 3,13
S-BIAD1518 Human kidney nephrectomy
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z_slices_analysed: 11,21
S-BIAD1518 Cleared mouse intestine
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z_slices_analysed: 14,24
S-BIAD1518 Synthetic cell nuclei
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z_slices_analysed: 58,68
S-BIAD1196 brain cell nucleus patches
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z_slices_analysed: 94,104
S-BIAD1026 Go-Nuclear image dataset
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z_slices_analysed: 126,136
S-BIAD1518 BABB-cleared kidney
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z_slices_analysed: 58,68
S-BIAD1518 Biopsy of diabetic human kidney
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z_slices_analysed: 11,21