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CHO cells nuclei virtual staining - brightfield - Pix2Pix

Estibaliz Gómez de Mariscal, Mario Del Rosario

Example image containing both original image data and after applying the model to it.

Model Information

Description: A conditional cycleGAN trained to infer the cell membrane as seen in a widefield fluorescence image from phase contrast images of CHO cells imaged with phase contrast microscopy. The cells are imaged with a 20x objective.
Task: prediction of cell membrane localisation
Licence: MIT
Tags: 2D, cells, in silico labeling, transmission light microscopy, fluorescence, conditional gan, cyclegan, zerocostdl4mic, deepimagej, pytorch, actin, dapi, nuclei

Benchmarking

This model was not tested on datasets with reference annotations, so we cannot produce benchmarking metrics.


Model predictions

Study Dataset Original image Prediction Analysis parameters
S-BIAD1269 CHO cell mitotic rounding raw brightfield microscopy images (for annotations)
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xy_image_crop_size: 1024,1024
S-BIAD1466 Nanoscale analysis of human G1 and metaphase chromatin in situ
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z_slices_analysed: 12,13
input_channel_analysed: 1
t_slices_analysed: 0,1