PDBsum entry 5hf2

Oxidoreductase

PDB id: 5hf2

Contents

Protein chain

157 a.a.

Ligands

XNP

U75

GOL

Waters ×120

PDB id:

5hf2

Name:

Oxidoreductase

Title:

Staphylococcus aureus dihydrofolate reductase complexed with cyclized alpha-NADPH anomer and 3'-(3-(2,4-diamino-6-ethylpyrimidin-5-yl)prop- 2-yn-1-yl)-5'-methoxy-[1,1'-biphenyl]-4-carboxylic acid (ucp1175)

Structure:

Dihydrofolate reductase. Chain: x. Synonym: dhfr. Engineered: yes

Source:

Staphylococcus aureus. Organism_taxid: 1280. Atcc: 43300. Gene: fola. Expressed in: escherichia coli. Expression_system_taxid: 469008.

Resolution:

1.81Å R-factor: 0.203 R-free: 0.232

Authors:

S.M.Reeve,A.C.Anderson

Key ref:

S.M.Reeve and a.c.anderson Staphylococcus aureus dihydrofolate reductase complex cyclized alpha-Nadph anomer and 3'-(3-(2,4-Diamino-6-Ethylpyrimidin-5-Yl)prop-2-Yn-1- -5'-Methoxy-[1,1'-Biphenyl]-4-Carboxylic acid (ucp117. To be published, .

Date:

06-Jan-16 Release date: 18-Jan-17

Protein chain

P0A017  (DYR_STAAU) -  Dihydrofolate reductase from Staphylococcus aureus

Seq: 159 a.a.

Struc: 157 a.a.

Enzyme reactions

• Enzyme class: E.C.1.5.1.3 - dihydrofolate reductase.
• Pathway: Folate Coenzymes
• Reaction: (6S)-5,6,7,8-tetrahydrofolate + NADP⁺ = 7,8-dihydrofolate + NADPH + H⁺

(6S)-5,6,7,8-tetrahydrofolate + NADP(+) (Bound ligand (Het Group name = XNP) corresponds exactly) = 7,8-dihydrofolate + NADPH + H(+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site