PDBsum entry 4c4k

Transferase

PDB id

4c4k

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Contents

			Protein chains

					94 a.a.


					97 a.a.

			Ligands

					EDO ×12

			Waters ×114

PDB id:

4c4k

Links

Name:

Transferase

Title:

Crystal structure of the titin m10-obscurin ig domain 1 complex

Structure:

Obscurin. Chain: o. Fragment: first ig domain, residues 9-103. Synonym: obscurin-rhogef, obscurin-myosin light chain kinase, obscurin-mlck. Engineered: yes. Titin. Chain: t. Fragment: m10 domain, residues 34252-34350.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Expressed in: escherichia coli. Expression_system_taxid: 469008. Expression_system_variant: rosetta 2.

Resolution:

1.95Å

R-factor:

0.173

R-free:

0.210

Authors:

S.Pernigo,A.Fukuzawa,M.Gautel,R.A.Steiner

Key ref:

S.Pernigo et al. (2015). The crystal structure of the human titin:obscurin complex reveals a conserved yet specific muscle M-band zipper module. J Mol Biol, 427, 718-736. PubMed id: 25490259 DOI: 10.1016/j.jmb.2014.11.019

Date:

05-Sep-13

Release date:

24-Sep-14

PROCHECK

	Headers

	References

Protein chain

Q5VST9 (OBSCN_HUMAN) - Obscurin from Homo sapiens

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Seq: Struc:					7968 a.a. 94 a.a.*

Protein chain

Q8WZ42 (TITIN_HUMAN) - Titin from Homo sapiens

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Seq: Struc:					34350 a.a. 97 a.a.

Key:

PfamA domain

Secondary structure

CATH domain

* PDB and UniProt seqs differ at 1 residue position (black cross)



		Enzyme reactions

Enzyme class:

Chains O, T: E.C.2.7.11.1 - non-specific serine/threonine protein kinase.

[IntEnz] [ExPASy] [KEGG] [BRENDA]

Reaction:

1.	L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H⁺
2.	L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H⁺

L-seryl-[protein]	+	ATP	=	O-phospho-L-seryl-[protein]	+	ADP	+	H(+)

L-threonyl-[protein]	+	ATP	=	O-phospho-L-threonyl-[protein]	+	ADP	+	H(+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

DOI no: 10.1016/j.jmb.2014.11.019	J Mol Biol 427:718-736 (2015)
PubMed id: 25490259

The crystal structure of the human titin:obscurin complex reveals a conserved yet specific muscle M-band zipper module.
S.Pernigo, A.Fukuzawa, A.Pandini, M.Holt, J.Kleinjung, M.Gautel, R.A.Steiner.

ABSTRACT

No abstract given.