PDBsum entry 3pmb

Hydrolase

PDB id: 3pmb

Contents

Protein chains

29 a.a.

237 a.a.

252 a.a.

Metals

_NA ×2

Waters ×74

PDB id:

3pmb

Name:

Hydrolase

Title:

2.9 angstrom crystal structure of bovine thrombin in tetragonal spacegroup

Structure:

Thrombin light chain. Chain: a, c. Fragment: bovine thrombin light chain residues 336-364. Thrombin heavy chain. Chain: b, d. Ec: 3.4.21.5

Source:

Bos taurus. Bovine,cow,domestic cattle,domestic cow. Organism_taxid: 9913. Organism_taxid: 9913

Resolution:

2.90Å R-factor: 0.245 R-free: 0.301

Authors:

H.T.Wright,J.N.Scarsdale,B.J.Desai

Key ref:

B.J.Desai et al. (2011). Interaction of thrombin with sucrose octasulfate. Biochemistry, 50, 6973-6982. PubMed id: 21736375

Date:

16-Nov-10 Release date: 20-Jul-11

Protein chains

P00735  (THRB_BOVIN) -  Prothrombin from Bos taurus

Seq: 625 a.a.

Struc: 29 a.a.

Protein chain

P00735  (THRB_BOVIN) -  Prothrombin from Bos taurus

Seq: 625 a.a.

Struc: 237 a.a.

Protein chain

P00735  (THRB_BOVIN) -  Prothrombin from Bos taurus

Seq: 625 a.a.

Struc: 252 a.a.

Enzyme reactions

• Enzyme class: Chains A, B, C, D: E.C.3.4.21.5 - thrombin.
• Reaction: Preferential cleavage: Arg-|-Gly; activates fibrinogen to fibrin and releases fibrinopeptide A and B.

Biochemistry 50:6973-6982 (2011)

PubMed id: 21736375

Interaction of thrombin with sucrose octasulfate.

B.J.Desai, R.S.Boothello, A.Y.Mehta, J.N.Scarsdale, H.T.Wright, U.R.Desai.

ABSTRACT

No abstract given.