PDBsum entry 3ogp

Hydrolase/hydrolase inhibitor

PDB id: 3ogp

Contents

Protein chains

112 a.a.

Ligands

017 ×2

DMS

Waters ×158

PDB id:

3ogp

Name:

Hydrolase/hydrolase inhibitor

Title:

Crystal structure of 6s-98s fiv protease with darunavir bound

Structure:

Fiv protease. Chain: a, b. Fragment: unp residues 39-154. Synonym: protease, retropepsin. Engineered: yes. Mutation: yes

Source:

Feline immunodeficiency virus. Fiv. Organism_taxid: 11673. Gene: pol, pr. Expressed in: escherichia coli. Expression_system_taxid: 469008.

Resolution:

1.70Å R-factor: 0.187 R-free: 0.229

Authors:

Y.-C.Lin,A.L.Perryman,J.H.Elder,C.D.Stout

Key ref:

Y.C.Lin et al. (2011). Structural basis for drug and substrate specificity exhibited by FIV encoding a chimeric FIV/HIV protease. Acta Crystallogr D Biol Crystallogr, 67, 540-548. PubMed id: 21636894

Date:

17-Aug-10 Release date: 08-Jun-11

Protein chains

P16088  (POL_FIVPE) -  Pol polyprotein from Feline immunodeficiency virus (isolate Petaluma)

Seq: 1124 a.a.

Struc: 112 a.a.*

* PDB and UniProt seqs differ at 6 residue positions (black crosses)

Enzyme reactions

• Enzyme class 2: E.C.2.7.7.49 - RNA-directed Dna polymerase.
• Reaction: DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate
• Enzyme class 3: E.C.3.1.13.2 - exoribonuclease H.
• Reaction: Exonucleolytic cleavage to 5'-phosphomonoester oligonucleotides in both 5'- to 3'- and 3'- to 5'-directions.
• Enzyme class 4: E.C.3.1.26.13 - retroviral ribonuclease H.
• Enzyme class 5: E.C.3.6.1.23 - dUTP diphosphatase.
• Reaction: dUTP + H2O = dUMP + diphosphate + H⁺

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

Acta Crystallogr D Biol Crystallogr 67:540-548 (2011)

PubMed id: 21636894

Structural basis for drug and substrate specificity exhibited by FIV encoding a chimeric FIV/HIV protease.

Y.C.Lin, A.L.Perryman, A.J.Olson, B.E.Torbett, J.H.Elder, C.D.Stout.

ABSTRACT

No abstract given.