PDBsum entry 3iby

Transport protein

PDB id: 3iby

Contents

Protein chains

245 a.a. *

230 a.a. *

Waters ×117

* Residue conservation analysis

PDB id:

3iby

Name:

Transport protein

Title:

Structure of cytosolic domain of l. Pneumophila feob

Structure:

Ferrous iron transport protein b. Chain: a, b, c, d. Fragment: cytosolic domain (unp residues 1-256). Engineered: yes

Source:

Legionella pneumophila. Organism_taxid: 446. Gene: feob. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

2.50Å R-factor: 0.216 R-free: 0.288

Authors:

N.Petermann,G.Hansen,R.Hilgenfeld

Key ref:

N.Petermann et al. (2010). Structure of the GTPase and GDI domains of FeoB, the ferrous iron transporter of Legionella pneumophila. Febs Lett, 584, 733-738. PubMed id: 20036663

Date:

17-Jul-09 Release date: 13-Oct-09

Protein chains

Q8GNS3  (FEOB_LEGPN) -  Fe(2+) transporter FeoB from Legionella pneumophila

Seq: 751 a.a.

Struc: 245 a.a.

Protein chain

Q8GNS3  (FEOB_LEGPN) -  Fe(2+) transporter FeoB from Legionella pneumophila

Seq: 751 a.a.

Struc: 230 a.a.

Febs Lett 584:733-738 (2010)

PubMed id: 20036663

Structure of the GTPase and GDI domains of FeoB, the ferrous iron transporter of Legionella pneumophila.

N.Petermann, G.Hansen, C.L.Schmidt, R.Hilgenfeld.

ABSTRACT

Prokaryotic pathogens have developed specialized mechanisms for efficient uptake of ferrous iron (Fe(2+)) from the host. In Legionella pneumophila, the causative agent of Legionnaires' disease, the transmembrane GTPase FeoB plays a key role in Fe(2+) acquisition and virulence. FeoB consists of a membrane-embedded core and an N-terminal, cytosolic region (NFeoB). Here, we report the crystal structure of NFeoB from L. pneumophila, revealing a monomeric protein comprising two separate domains with GTPase and guanine-nucleotide dissociation inhibitor (GDI) functions. The GDI domain displays a novel fold, whereas the overall structure of the GTPase domain resembles that of known G domains but is in the rarely observed nucleotide-free state.