PDBsum entry 2x4f

Transferase

PDB id: 2x4f

Contents

Protein chain

289 a.a. *

Ligands

16X ×2

SO4

1PE

EDO ×3

Waters ×252

* Residue conservation analysis

PDB id:

2x4f

Name:

Transferase

Title:

The crystal structure of the human myosin light chain kinase loc340156.

Structure:

Myosin light chain kinase family member 4. Chain: a, b. Fragment: light chain, residues 40-388. Synonym: serine/threonine-protein kinase sgk085, sugen kinase 85. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Expression_system_cell_line: sf9.

Resolution:

2.67Å R-factor: 0.193 R-free: 0.241

Authors:

J.R.C.Muniz,P.Mahajan,P.Rellos,O.Fedorov,B.Shrestha,J.Wang, J.M.Elkins,N.Daga,R.Cocking,A.Chaikuad,T.Krojer,E.Ugochukwu,W.Yue, F.Von Delft,C.H.Arrowsmith,A.M.Edwards,J.Weigelt,C.Bountra, O.Gileadi,S.Knapp

Key ref:

J.R.C.Muniz et al. The crystal structure of the human myosin light chain kinase loc340156. To be published, .

Date:

29-Jan-10 Release date: 09-Feb-10

Protein chains

Q86YV6  (MYLK4_HUMAN) -  Myosin light chain kinase family member 4 from Homo sapiens

Seq: 388 a.a.

Struc: 289 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.2.7.11.1 - non-specific serine/threonine protein kinase.
• Reaction:
  1. L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H⁺
  2. L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H⁺

Molecule diagrams generated from .mol files obtained from the KEGG ftp site