PDBsum entry 2lu1

Hydrolase

PDB id: 2lu1

Contents

Protein chain

89 a.a.

PDB id:

2lu1

Name:

Hydrolase

Title:

Pfsub2 solution nmr structure

Structure:

Subtilase. Chain: a. Fragment: sheddase inhibitor prodomain. Engineered: yes

Source:

Plasmodium falciparum. Organism_taxid: 5833. Gene: sub-2. Expressed in: escherichia coli. Expression_system_taxid: 562.

NMR struc:

20 models

Authors:

Y.He,Y.Chen,B.Ruan,D.O'Brochta,P.Bryan,J.Orban

Key ref:

Y.He et al. (2012). Solution NMR structure of a sheddase inhibitor prodomain from the malarial parasite Plasmodium falciparum. Proteins, 80, 2810-2817. PubMed id: 23011838

Date:

06-Jun-12 Release date: 03-Oct-12

Protein chain

O97364  (SUB2_PLAFA) -  Subtilisin-like protease 2 from Plasmodium falciparum

Seq: 1342 a.a.

Struc: 89 a.a.

Enzyme reactions

• Enzyme class: E.C.3.4.21.62 - subtilisin.
• Reaction: Hydrolysis of proteins with broad specificity for peptide bonds, and a preference for a large uncharged residue in P1. Hydrolyzes peptide amides.

Proteins 80:2810-2817 (2012)

PubMed id: 23011838

Solution NMR structure of a sheddase inhibitor prodomain from the malarial parasite Plasmodium falciparum.

Y.He, Y.Chen, N.Oganesyan, B.Ruan, D.O'Brochta, P.N.Bryan, J.Orban.

ABSTRACT

Plasmodium subtilisin 2 (Sub2) is a multidomain protein that plays an important role in malaria infection. Here, we describe the solution NMR structure of a conserved region of the inhibitory prodomain of Sub2 from Plasmodium falciparum, termed prosub2. Despite the absence of any detectable sequence homology, the protozoan prosub2 has structural similarity to bacterial and mammalian subtilisin-like prodomains. Comparison with the three-dimensional structures of these other prodomains suggests a likely binding interface with the catalytic domain of Sub2 and provides insights into the locations of primary and secondary processing sites in Plasmodium prodomains. Proteins 2012;. © 2012 Wiley Periodicals, Inc.