_EC 3.4.21.62 Subtilisin. 105 PDB entries  
EC 3.-.-.- Hydrolases. [21,057 PDB entries]
EC 3.4.-.- Acting on peptide bonds (peptide hydrolases). [6,846 PDB entries]
EC 3.4.21.- Serine endopeptidases. [2,535 PDB entries]
EC 3.4.21.62 Subtilisin. [105 PDB entries]    
1a10

Reaction: Hydrolysis of proteins with broad specificity for peptide bonds, and a preference for a large uncharged residue in P1. Hydrolyzes peptide amides.
Comments: Subtilisin is a serine endopeptidase that evolved independently of chymotrypsin. It contains no cysteine residues (although these are found in homologous enzymes). Species variants include subtilisin BPN' (also subtilisin B, subtilopeptidase C, nagarse, nagarse proteinase, subtilisin Novo, bacterial proteinase Novo) and subtilisin Carlsberg (subtilisin A, subtilopeptidase A, alcalase Novo). Similar enzymes are produced by various Bacillus subtilis strains and other Bacillus species.
Links:   [IntEnz]   [ExPASy]   [KEGG]  

There are 105 PDB entries in enzyme class E.C.3.4.21.62

  PDB code Protein
1a10
Alcalase (subtilisin carlsberg variant) in complex with chymotrypsin inhibitor ci-2a(m59p)
Source: Bacillus licheniformis. Expressed in: null. Hordeum vulgare. Barley. Expressed in: null
Chain: E (274 residues) CATH domain: 3.40.50.200
1a1y
Alcalase (subtilisin carlsberg variant) in complex with chymotrypsin inhibitor ci-2a
Source: Bacillus licheniformis. Expressed in: null. Hordeum vulgare. Barley. Expressed in: null
Chain: E (274 residues) CATH domain: 3.40.50.200
1a2q
Subtilisin bpn' mutant 7186
Source: Bacillus amyloliquefaciens. Organism_taxid: 1390. Strain: gx7186. Cellular_location: secreted. Expressed in: bacillus subtilis. Expression_system_taxid: 1423.
Chain: A (275 residues) CATH domain: 3.40.50.200
1af4
Crystal structure of subtilisin carlsberg in anhydrous dioxane
Source: Bacillus licheniformis. Organism_taxid: 1402
Chain: A (274 residues) CATH domain: 3.40.50.200
1ak9
Subtilisin mutant 8321
Source: Bacillus amyloliquefaciens. Organism_taxid: 1390. Expressed in: bacillus subtilis. Expression_system_taxid: 1423.
Chain: A (275 residues) CATH domain: 3.40.50.200
1aqn
Subtilisin mutant 8324
Source: Bacillus amyloliquefaciens. Organism_taxid: 1390. Strain: gx8324. Cellular_location: secreted. Expressed in: bacillus subtilis. Expression_system_taxid: 1423.
Chain: A (275 residues) CATH domain: 3.40.50.200
1au9
Subtilisin bpn' mutant 8324 in citrate
Source: Bacillus amyloliquefaciens. Organism_taxid: 1390. Strain: gx8324. Cellular_location: secreted. Expressed in: bacillus subtilis. Expression_system_taxid: 1423.
Chain: A (275 residues) CATH domain: 3.40.50.200
1av7
Subtilisin carlsberg l-naphthyl-1-acetamido boronic acid inhibitor complex
Source: Bacillus licheniformis. Organism_taxid: 1402. Other_details: purchased from sigma
Chain: A (274 residues) CATH domain: 3.40.50.200
1avt
Subtilisin carlsberg d-para-chlorophenyl-1-acetamido boronic acid inhibitor complex
Source: Bacillus licheniformis. Organism_taxid: 1402. Other_details: purchased from sigma
Chain: A (274 residues) CATH domain: 3.40.50.200
1be6
Trans-cinnamoyl-subtilisin in anhydrous acetonitrile
Source: Bacillus licheniformis. Organism_taxid: 1402
Chain: A (274 residues) CATH domain: 3.40.50.200
1be8
Trans-cinnamoyl-subtilisin in water
Source: Bacillus licheniformis. Organism_taxid: 1402
Chain: A (274 residues) CATH domain: 3.40.50.200
1bfk
Crystal structure of subtilisin carlsberg in 40% acetonitrile
Source: Bacillus licheniformis. Organism_taxid: 1402
Chain: A (274 residues) CATH domain: 3.40.50.200
1bfu
Subtilisin carlsberg in 20% dioxane
Source: Bacillus licheniformis. Organism_taxid: 1402
Chain: A (274 residues) CATH domain: 3.40.50.200
1bh6
Subtilisin dy in complex with the synthetic inhibitor n- benzyloxycarbonyl-ala-pro-phe-chloromethyl ketone
Source: Bacillus licheniformis. Organism_taxid: 1402. Variant: dy. Other_details: x-irradiated japanese strain of bacillus licheniformis
Chain: A (274 residues) CATH domain: 3.40.50.200
1c13
Structure on native (asn 87) subtilisin from bacillus lentus
Source: Bacillus lentus. Bacteria. Expressed in: e. Coli.
Chain: A (269 residues) CATH domain: 3.40.50.200
1c3l
Subtilisin-carlsberg complexed with xenon (8 bar)
Source: Bacillus licheniformis. Organism_taxid: 1402
Chain: A (274 residues) CATH domain: 3.40.50.200
1c9j
Bacillus lentus subtilisin k27r/n87s/v104y/n123s/t274a variant
Source: Bacillus lentus. Organism_taxid: 1467. Expressed in: bacillus subtilis. Expression_system_taxid: 1423
Chain: A (269 residues) CATH domain: 3.40.50.200
1c9m
Bacillus lentus subtilsin (ser 87) n76d/s103a/v104i
Source: Bacillus lentus. Organism_taxid: 1467. Expressed in: bacillus subtilis. Expression_system_taxid: 1423
Chain: A (269 residues) CATH domain: 3.40.50.200
1c9n
Bacillus lentus substilisin variant (ser 87) k27r/v104y/n123s/t274a
Source: Bacillus lentus. Organism_taxid: 1467. Expressed in: bacillus subtilis. Expression_system_taxid: 1423
Chain: A (269 residues) CATH domain: 3.40.50.200
1cse
The high-resolution x-ray crystal structure of the complex formed between subtilisin carlsberg and eglin c, an elastase inhibitor from the leech hirudo medicinalis. Structural analysis, subtilisin structure and interface geometry
Source: Bacillus subtilis. Organism_taxid: 1423. Hirudo medicinalis. Medicinal leech. Organism_taxid: 6421
Chain: E (274 residues) CATH domain: 3.40.50.200
1dui
Subtilisin bpn' from bacillus amyloliquefaciens, crystal growth mutant
Source: Bacillus amyloliquefaciens. Organism_taxid: 1390. Cellular_location: secreted. Expressed in: bacillus amyloliquefaciens. Expression_system_taxid: 1390.
Chain: A (266 residues) CATH domain: 3.40.50.200
1gci
The 0.78 angstroms structure of a serine protease-bacillus l subtilisin
Source: Bacillus lentus. Organism_taxid: 1467. Expressed in: bacillus subtilis. Expression_system_taxid: 1423
Chain: A (269 residues) CATH domain: 3.40.50.200
1gns
Subtilisin bpn'
Source: Bacillus amyloliquefaciens. Organism_taxid: 1390. Expressed in: escherichia coli. Expression_system_taxid: 469008.
Chain: A (263 residues) CATH domain: 3.40.50.200
1gnv
Calcium independent subtilisin bpn' mutant
Source: Bacillus amyloliquefaciens. Organism_taxid: 1390. Expressed in: escherichia coli. Expression_system_taxid: 562
Chain: A (266 residues) CATH domain: 3.40.50.200
1iav
Structure on native (asn 87) subtilisin from bacillus lentus
Source: Bacillus lentus. Organism_taxid: 1467. Expressed in: escherichia coli. Expression_system_taxid: 562
Chain: A (269 residues) CATH domain: 3.40.50.200
1jea
Altered topology and flexibility in engineered subtilisin
Source: Bacillus lentus. Organism_taxid: 1467. Expressed in: bacillus subtilis. Expression_system_taxid: 1423
Chain: A (269 residues) CATH domain: 3.40.50.200
1lw6
Crystal structure of the complex of subtilisin bpn' with chymotrypsin inhibitor 2 at 1.5 angstrom resolution
Source: Bacillus amyloliquefaciens. Organism_taxid: 1390. Expressed in: bacillus subtilis. Expression_system_taxid: 1423. Hordeum vulgare. Organism_taxid: 4513. Strain: hiproly. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
Chain: E (281 residues) CATH domain: 3.40.50.200
1mee
The complex between the subtilisin from a mesophilic bacterium and the leech inhibitor eglin-c
Source: Bacillus pumilus. Organism_taxid: 1408. Hirudo medicinalis. Medicinal leech. Organism_taxid: 6421
Chain: A (275 residues) CATH domain: 3.40.50.200
1ndq
Bacillus lentus subtilisin
Source: Bacillus lentus. Organism_taxid: 1467
Chain: A (269 residues) CATH domain: 3.40.50.200
1ndu
Bacillus lentus subtilisin variant s101g/v104n
Source: Bacillus lentus. Organism_taxid: 1467
Chain: A (269 residues) CATH domain: 3.40.50.200
1oyv
Crystal structure of tomato inhibitor-ii in a ternary complex with subtilisin carlsberg
Source: Bacillus licheniformis. Organism_taxid: 1402. Strain: nagarse. Solanum lycopersicum. Organism_taxid: 4081. Strain: bonny best. Other_details: leaves of tomato plant
Chains: A, B (274 residues) CATH domain: 3.40.50.200
1q5p
S156e/s166d variant of bacillus lentus subtilisin
Source: Bacillus lentus. Organism_taxid: 1467. Expressed in: bacillus subtilis. Expression_system_taxid: 1423
Chain: A (269 residues) CATH domain: 3.40.50.200
1r0r
1.1 angstrom resolution structure of the complex between the protein inhibitor, omtky3, and the serine protease, subtilisin carlsberg
Source: Bacillus licheniformis. Organism_taxid: 1402. Meleagris gallopavo. Turkey. Organism_taxid: 9103
Chain: E (274 residues) CATH domain: 3.40.50.200
1s01
Large increases in general stability for subtilisin bpn(prime) through incremental changes in the free energy of unfolding
Source: Bacillus amyloliquefaciens. Organism_taxid: 1390
Chain: A (275 residues) CATH domain: 3.40.50.200
1s02
Effects of engineered salt bridges on the stability of subtilisin bpn'
Source: Bacillus amyloliquefaciens. Organism_taxid: 1390
Chain: A (275 residues) CATH domain: 3.40.50.200
1sbc
The refined crystal structure of subtilisin carlsberg at 2.5 angstroms resolution
Source: Bacillus subtilis. Organism_taxid: 1423
Chain: A (274 residues) CATH domain: 3.40.50.200
1sbh
Subtilisin bpn' 8397+1 (E.C. 3.4.21.14) mutant (m50f, n76d, g169a, q206c, n218s, k256y)
Source: Bacillus amyloliquefaciens. Organism_taxid: 1390. Gene: 8397+1. Expressed in: bacillus subtilis. Expression_system_taxid: 1423.
Chain: A (275 residues) CATH domain: 3.40.50.200
1sbi
Subtilisin bpn' 8397 (E.C. 3.4.21.14) mutant (m50f, n76d, g169a, q206c, n218s)
Source: Bacillus amyloliquefaciens. Organism_taxid: 1390. Gene: 8397. Expressed in: bacillus subtilis. Expression_system_taxid: 1423.
Chain: A (275 residues) CATH domain: 3.40.50.200
1sbn
Refined crystal structures of subtilisin novo in complex with wild-type and two mutant eglins. Comparison with other serine proteinase inhibitor complexes
Source: Bacillus subtilis. Organism_taxid: 1423. Hirudo medicinalis. Medicinal leech. Organism_taxid: 6421. Expressed in: escherichia coli. Expression_system_taxid: 562
Chain: E (275 residues) CATH domain: 3.40.50.200
1sbt
Atomic coordinates for subtilisin bpn (or novo)
Source: Bacillus amyloliquefaciens. Organism_taxid: 1390
Chain: A (275 residues) CATH domain: 3.40.50.200
1sca
Enzyme crystal structure in a neat organic solvent
Source: Bacillus licheniformis. Organism_taxid: 1402
Chain: A (274 residues) CATH domain: 3.40.50.200
1scb
Enzyme crystal structure in a neat organic solvent
Source: Bacillus licheniformis. Organism_taxid: 1402
Chain: A (274 residues) CATH domain: 3.40.50.200
1scd
X-ray crystal structure of cross-linked subtilism carlsberg in water vs. Acetonitrile
Source: Bacillus licheniformis. Organism_taxid: 1402
Chain: A (274 residues) CATH domain: 3.40.50.200
1scj
Crystal structure of subtilisin-propeptide complex
Source: Bacillus subtilis. Organism_taxid: 1423. Strain: 168. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (275 residues) CATH domain: 3.40.50.200
1scn
Inactivation of subtilisin carlsberg by n-(tert-butoxycarbon prolyl-phenylalanyl)-o-benzol hydroxylamine: formation of c enzyme-inhibitor linkage in the form of a carbamate derivat
Source: Bacillus licheniformis. Organism_taxid: 1402
Chain: E (274 residues) CATH domain: 3.40.50.200
1sel
Crystal structure of selenosubtilisin at 2.0-angstroms resol
Source: Bacillus subtilis. Organism_taxid: 1423
Chains: A, B (274 residues) CATH domain: 3.40.50.200
1sib
Refined crystal structures of subtilisin novo in complex with wild-type and two mutant eglins. Comparison with other serine proteinase inhibitor complexes
Source: Bacillus subtilis. Organism_taxid: 1423. Hirudo medicinalis. Medicinal leech. Organism_taxid: 6421. Expressed in: escherichia coli. Expression_system_taxid: 562
Chain: E (275 residues) CATH domain: 3.40.50.200
1spb
Subtilisin bpn' prosegment (77 residues) complexed with a mutant subtilisin bpn' (266 residues). Crystal ph 4.6. Crystallization temperature 20 c diffraction temperature- 160 c
Source: Bacillus amyloliquefaciens. Organism_taxid: 1390. Expressed in: escherichia coli. Expression_system_taxid: 562. Expression_system_taxid: 562
Chains: P, S (71 residues) CATH domain: 3.30.70.80
1st2
The three-dimensional structure of bacillus amyloliquefaciens subtilisin at 1.8 angstroms and an analysis of the structural consequences of peroxide inactivation
Source: Bacillus amyloliquefaciens. Organism_taxid: 1390. Expressed in: escherichia coli. Expression_system_taxid: 562
Chain: A (275 residues) CATH domain: 3.40.50.200
1st3
The crystal structure of the bacillus lentus alkaline protease, subtilisin bl, at 1.4 angstroms resolution
Source: Bacillus lentus. Organism_taxid: 1467
Chain: A (269 residues) CATH domain: 3.40.50.200
1sua
Subtilisin bpn'
Source: Bacillus amyloliquefaciens. Organism_taxid: 1390. Cell_line: bl21. Cellular_location: extracellular. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
Chain: A (263 residues) CATH domain: 3.40.50.200
1sub
Calcium-independent subtilisin by design
Source: Bacillus amyloliquefaciens. Organism_taxid: 1390
Chain: A (275 residues) CATH domain: 3.40.50.200
1suc
Calcium-independent subtilisin by design
Source: Bacillus amyloliquefaciens. Organism_taxid: 1390
Chain: A (263 residues) CATH domain: 3.40.50.200
1sud
Calcium-independent subtilisin by design
Source: Bacillus amyloliquefaciens. Organism_taxid: 1390
Chain: A (275 residues) CATH domain: 3.40.50.200
1sue
Subtilisin bpn' from bacillus amyloliquefaciens, mutant
Source: Bacillus amyloliquefaciens. Organism_taxid: 1390. Cellular_location: secreted. Expressed in: bacillus amyloliquefaciens. Expression_system_taxid: 1390.
Chain: A (266 residues) CATH domain: 3.40.50.200
1sup
Subtilisin bpn' at 1.6 angstroms resolution: analysis of discrete disorder and comparison of crystal forms
Source: Bacillus amyloliquefaciens. Organism_taxid: 1390
Chain: A (275 residues) CATH domain: 3.40.50.200
1svn
Savinase
Source: Bacillus lentus. Organism_taxid: 1467
Chain: A (269 residues) CATH domain: 3.40.50.200
1tk2
Crystal structure of the complex formed between alkaline pro savinase and gramicidin s at 1.5a resolution
Source: Bacillus lentus. Organism_taxid: 1467. Synthetic: yes. Brevibacillus brevis. Organism_taxid: 1393
Chain: A (269 residues) CATH domain: 3.40.50.200
1tm1
Crystal structure of the complex of subtilisin bpn' with chymotrypsin inhibitor 2
Source: Bacillus amyloliquefaciens. Organism_taxid: 1390. Gene: apr. Expressed in: bacillus subtilis. Expression_system_taxid: 1423. Hordeum vulgare subsp. Vulgare. Domesticated barley. Organism_taxid: 112509. Strain: subsp. Vulgare.
Chain: E (281 residues) CATH domain: 3.40.50.200
1tm3
Crystal structure of the complex of subtilisin bpn' with chymotrypsin inhibitor 2 m59k mutant
Source: Bacillus amyloliquefaciens. Organism_taxid: 1390. Gene: apr. Expressed in: bacillus subtilis. Expression_system_taxid: 1423. Hordeum vulgare subsp. Vulgare. Domesticated barley. Organism_taxid: 112509. Strain: subsp. Vulgare.
Chain: E (281 residues) CATH domain: 3.40.50.200
1tm4
Crystal structure of the complex of subtilsin bpn'with chymotrypsin inhibitor 2 m59g mutant
Source: Bacillus amyloliquefaciens. Organism_taxid: 1390. Gene: apr. Expressed in: bacillus subtilis. Expression_system_taxid: 1423. Hordeum vulgare subsp. Vulgare. Domesticated barley. Organism_taxid: 112509. Strain: subsp. Vulgare.
Chain: E (281 residues) CATH domain: 3.40.50.200
1tm5
Crystal structure of the complex of subtilisin bpn' with chymotrypsin inhibitor 2 m59a mutant
Source: Bacillus amyloliquefaciens. Organism_taxid: 1390. Gene: apr. Expressed in: bacillus subtilis. Expression_system_taxid: 1423. Hordeum vulgare subsp. Vulgare. Domesticated barley. Organism_taxid: 112509. Strain: subsp. Vulgare.
Chain: E (281 residues) CATH domain: 3.40.50.200
1tm7
Crystal structure of the complex of subtilisin bpn' with chymotrypsin inhibitor 2 m59y mutant
Source: Bacillus amyloliquefaciens. Organism_taxid: 1390. Gene: apr. Expressed in: bacillus subtilis. Expression_system_taxid: 1423. Hordeum vulgare subsp. Vulgare. Domesticated barley. Organism_taxid: 112509. Strain: subsp. Vulgare.
Chain: E (281 residues) CATH domain: 3.40.50.200
1tmg
Crystal structure of the complex of subtilisin bpn' with chymotrypsin inhibitor 2 m59f mutant
Source: Bacillus amyloliquefaciens. Organism_taxid: 1390. Gene: apr. Expressed in: bacillus subtilis. Expression_system_taxid: 1423. Hordeum vulgare subsp. Vulgare. Domesticated barley. Organism_taxid: 112509. Strain: subsp. Vulgare.
Chain: E (281 residues) CATH domain: 3.40.50.200
1to1
Crystal structure of the complex of subtilisin bpn' with chymotrypsin inhibitor 2 y61a mutant
Source: Bacillus amyloliquefaciens. Organism_taxid: 1390. Gene: apr. Expressed in: bacillus subtilis. Expression_system_taxid: 1423. Hordeum vulgare subsp. Vulgare. Domesticated barley. Organism_taxid: 112509. Strain: subsp. Vulgare.
Chain: E (281 residues) CATH domain: 3.40.50.200
1to2
Crystal structure of the complex of subtilisin bpn' with chymotrypsin inhibitor 2 m59k, in ph 9 cryosoak
Source: Bacillus amyloliquefaciens. Organism_taxid: 1390. Gene: apr. Expressed in: bacillus subtilis. Expression_system_taxid: 1423. Hordeum vulgare subsp. Vulgare. Domesticated barley. Organism_taxid: 112509. Strain: subsp. Vulgare.
Chain: E (281 residues) CATH domain: 3.40.50.200
1ubn
Selenosubtilisin bpn
Source: Bacillus amyloliquefaciens. Organism_taxid: 1390. Expressed in: bacillus subtilis. Expression_system_taxid: 1423
Chain: A (275 residues) CATH domain: 3.40.50.200
1v5i
Crystal structure of serine protease inhibitor poia1 in comp subtilisin bpn'
Source: Bacillus amyloliquefaciens. Organism_taxid: 1390. Expressed in: escherichia coli. Expression_system_taxid: 562. Pleurotus ostreatus. Oyster mushroom. Organism_taxid: 5322. Gene: poia1. Expression_system_taxid: 562
Chain: A (275 residues) CATH domain: 3.40.50.200
1vsb
Subtilisin carlsberg l-para-chlorophenyl-1-acetamido boronic acid inhibitor complex
Source: Bacillus licheniformis. Organism_taxid: 1402. Other_details: purchased from sigma
Chain: A (274 residues) CATH domain: 3.40.50.200
1y1k
Crystal structure of the complex of subtilisin bpn' with chymotrypsin inhibitor 2 t58a mutant
Source: Bacillus amyloliquefaciens. Organism_taxid: 1390. Gene: apr. Expressed in: bacillus subtilis. Expression_system_taxid: 1423. Hordeum vulgare. Organism_taxid: 4513. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
Chain: E (281 residues) CATH domain: 3.40.50.200
1y33
Crystal structure of the complex of subtilisin bpn' with chymotrypsin inhibitor 2 t58p mutant
Source: Bacillus amyloliquefaciens. Organism_taxid: 1390. Gene: apr. Expressed in: bacillus subtilis. Expression_system_taxid: 1423. Hordeum vulgare. Organism_taxid: 4513. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
Chain: E (281 residues) CATH domain: 3.40.50.200
1y34
Crystal structure of the complex of subtilisin bpn' with chymotrypsin inhibitor 2 e60a mutant
Source: Bacillus amyloliquefaciens. Organism_taxid: 1390. Gene: apr. Expressed in: bacillus subtilis. Expression_system_taxid: 1423. Hordeum vulgare. Organism_taxid: 4513. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
Chain: E (281 residues) CATH domain: 3.40.50.200
1y3b
Crystal structure of the complex of subtilisin bpn' with chymotrypsin inhibitor 2 e60s mutant
Source: Bacillus amyloliquefaciens. Organism_taxid: 1390. Gene: apr. Expressed in: bacillus subtilis. Expression_system_taxid: 1423. Hordeum vulgare. Organism_taxid: 4513. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
Chain: E (281 residues) CATH domain: 3.40.50.200
1y3c
Crystal structure of the complex of subtilisin bpn' with chymotrypsin inhibitor 2 r62a mutant
Source: Bacillus amyloliquefaciens. Organism_taxid: 1390. Gene: apr. Expressed in: bacillus subtilis. Expression_system_taxid: 1423. Hordeum vulgare. Organism_taxid: 4513. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
Chain: E (281 residues) CATH domain: 3.40.50.200
1y3d
Crystal structure of the complex of subtilisin bpn' with chymotrypsin inhibitor 2 r67a mutant
Source: Bacillus amyloliquefaciens. Organism_taxid: 1390. Gene: apr. Expressed in: bacillus subtilis. Expression_system_taxid: 1423. Hordeum vulgare. Organism_taxid: 4513. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
Chain: E (281 residues) CATH domain: 3.40.50.200
1y3f
Crystal structure of the complex of subtilisin bpn' with chymotrypsin inhibitor 2 f69a mutant
Source: Bacillus amyloliquefaciens. Organism_taxid: 1390. Gene: apr. Expressed in: bacillus subtilis. Expression_system_taxid: 1423. Hordeum vulgare. Organism_taxid: 4513. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
Chain: E (281 residues) CATH domain: 3.40.50.200
1y48
Crystal structure of the complex of subtilisin bpn' with chymotrypsin inhibitor 2 r65a mutant
Source: Bacillus amyloliquefaciens. Organism_taxid: 1390. Gene: apr. Expressed in: bacillus subtilis. Expression_system_taxid: 1423. Hordeum vulgare. Organism_taxid: 4513. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
Chain: E (281 residues) CATH domain: 3.40.50.200
1y4a
Crystal structure of the complex of subtilisin bpn' with chymotrypsin inhibitor 2 m59r/e60s mutant
Source: Bacillus amyloliquefaciens. Organism_taxid: 1390. Gene: apr. Expressed in: bacillus subtilis. Expression_system_taxid: 1423. Hordeum vulgare. Organism_taxid: 4513. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
Chain: E (275 residues) CATH domain: 3.40.50.200
1y4d
Crystal structure of the complex of subtilisin bpn' with chymotrypsin inhibitor 2 m59r/e60s mutant
Source: Bacillus amyloliquefaciens. Organism_taxid: 1390. Gene: apr. Expressed in: bacillus subtilis. Expression_system_taxid: 1423. Hordeum vulgare. Organism_taxid: 4513. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
Chain: E (276 residues) CATH domain: 3.40.50.200
1yja
Subtilisin bpn' 8397+1 (E.C. 3.4.21.14) (mutant with met 50 replaced by phe, asn 76 replaced by asp, gly 169 replaced by ala, gln 206 replaced by cys, asn 218 replaced by ser and lys 256 replaced by tyr) (m50f, n76d, g169a, q206c, n218s, and k256y) in 20% dimethylformamide
Source: Bacillus amyloliquefaciens. Organism_taxid: 1390. Gene: 8397+1. Expressed in: bacillus subtilis. Expression_system_taxid: 1423.
Chain: A (275 residues) CATH domain: 3.40.50.200
1yjb
Subtilisin bpn' 8397+1 (E.C. 3.4.21.14) (mutant with met 50 replaced by phe, asn 76 replaced by asp, gly 169 replaced by ala, gln 206 replaced by cys, asn 218 replaced by ser and lys 256 replaced by tyr) (m50f, n76d, g169a, q206c, n218s, and k256y) in 35% dimethylformamide
Source: Bacillus amyloliquefaciens. Organism_taxid: 1390. Gene: 8397+1. Expressed in: bacillus subtilis. Expression_system_taxid: 1423.
Chain: A (275 residues) CATH domain: 3.40.50.200
1yjc
Subtilisin bpn' 8397+1 (E.C. 3.4.21.14) (mutant with met 50 replaced by phe, asn 76 replaced by asp, gly 169 replaced by ala, gln 206 replaced by cys, asn 218 replaced by ser and lys 256 replaced by tyr) (m50f, n76d, g169a, q206c, n218s, and k256y) in 50% dimethylformamide
Source: Bacillus amyloliquefaciens. Organism_taxid: 1390. Gene: 8397+1. Expressed in: bacillus subtilis. Expression_system_taxid: 1423.
Chain: A (275 residues) CATH domain: 3.40.50.200
1yu6
Crystal structure of the subtilisin carlsberg:omtky3 complex
Source: Bacillus licheniformis. Organism_taxid: 1402. Meleagris gallopavo. Turkey. Organism_taxid: 9103. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (274 residues) CATH domain: 3.40.50.200
2sbt
A comparison of the three-dimensional structures of subtilisin bpn and subtilisin novo
Source: Bacillus amyloliquefaciens. Organism_taxid: 1390
Chain: A (275 residues) CATH domain: 3.40.50.200
2sec
Structural comparison of two serine proteinase-protein inhibitor complexes. Eglin-c-subtilisin carlsberg and ci-2- subtilisin novo
Source: Bacillus licheniformis. Organism_taxid: 1402. Hirudo medicinalis. Medicinal leech. Organism_taxid: 6421. Expressed in: unidentified. Expression_system_taxid: 32644
Chain: E (274 residues) CATH domain: 3.40.50.200
2sic
Refined crystal structure of the complex of subtilisin bpn' and streptomyces subtilisin inhibitor at 1.8 angstroms resolution
Source: Bacillus amyloliquefaciens. Organism_taxid: 1390.
Chain: E (275 residues) CATH domain: 3.40.50.200
2sni
Structural comparison of two serine proteinase-protein inhibitor complexes. Eglin-c-subtilisin carlsberg and ci-2- subtilisin novo
Source: Bacillus amyloliquefaciens. Organism_taxid: 1390. Hordeum sp.. Organism_taxid: 50472
Chain: E (275 residues) CATH domain: 3.40.50.200
2st1
The three-dimensional structure of bacillus amyloliquefaciens subtilisin at 1.8 angstroms and an analysis of the structural consequences of peroxide inactivation
Source: Bacillus amyloliquefaciens. Organism_taxid: 1390
Chain: A (275 residues) CATH domain: 3.40.50.200
2wuv
Crystallographic analysis of counter-ion effects on subtilisin enzymatic action in acetonitrile
Source: Bacillus licheniformis. Organism_taxid: 1402
Chain: A (274 residues) CATH domain: 3.40.50.200
2wuw
Crystallographic analysis of counter-ion effects on subtilisin enzymatic action in acetonitrile (native data)
Source: Bacillus licheniformis. Organism_taxid: 1402
Chain: E (274 residues) CATH domain: 3.40.50.200
3bgo
Azide complex of engineered subtilisin subt_bacam
Source: Bacillus amyloliquefaciens. Gene: apr. Expressed in: eschericia coli. Expressed in: eschericia coli
Chains: P, S (71 residues) CATH domain: 3.30.70.80
3bx1
Complex between the barley alpha-amylase/subtilisin inhibitor and the subtilisin savinase
Source: Bacillus lentus. Expressed in: bacillus subtilis. Hordeum vulgare. Barley. Expressed in: pichia pastoris
Chains: A, B (269 residues) CATH domain: 3.40.50.200
3cnq
Prosubtilisin substrate complex of subtilisin subt_bacam
Source: Bacillus amyloliquefaciens. Organism_taxid: 1390. Gene: apr. Expressed in: escherichia coli. Expression_system_taxid: 469008.
Chains: P, S (71 residues) CATH domain: 3.30.70.80
3co0
Substrate complex of fluoride-sensitive engineered subtilisin subt_bacam
Source: Bacillus amyloliquefaciens. Organism_taxid: 1390. Gene: apr. Expressed in: eschericia coli. Expressed in: eschericia coli
Chains: P, S (71 residues) CATH domain: 3.30.70.80
3f49
Anion-triggered engineered subtilisin subt_bacam
Source: Bacillus amyloliquefaciens. Organism_taxid: 1390. Gene: apr, subt_bacam. Expressed in: eschericia coli
Chain: S (266 residues) CATH domain: 3.40.50.200
3sic
Molecular recognition at the active site of subtilisin bpn': crystallographic studies using genetically engineered proteinaceous inhibitor ssi (streptomyces subtilisin inhibitor)
Source: Bacillus amyloliquefaciens. Organism_taxid: 1390.
Chain: E (275 residues) CATH domain: 3.40.50.200
3unx
Bond length analysis of asp, glu and his residues in subtili carlsberg at 1.26a resolution
Source: Bacillus licheniformis. Organism_taxid: 1402. Gene: apr. Expressed in: escherichia coli. Expression_system_taxid: 562
Chain: A (274 residues) CATH domain: 3.40.50.200
3vsb
Subtilisin carlsberg d-naphthyl-1-acetamido boronic acid inhibitor complex
Source: Bacillus licheniformis. Organism_taxid: 1402. Other_details: purchased from sigma
Chain: A (274 residues) CATH domain: 3.40.50.200
3vyv
Crystal structure of subtilisin nat at 1.36
Source: Bacillus subtilis subsp. Natto. Organism_taxid: 86029. Strain: subsp. Natto
Chains: A, B (275 residues) CATH domain: 3.40.50.200
3whi
Crystal structure of unautoprocessed form of is1-inserted pr subtilisin e
Source: Bacillus subtilis subsp. Subtilis. Organism_taxid: 224308. Strain: 168. Gene: apre, apr, apra, spre, bsu10300. Expressed in: escherichia coli. Expression_system_taxid: 562.
Chains: A, B (355 residues) CATH domains: 3.30.70.80 3.40.50.200
4cfy
Savinase crystal structures for combined single crystal diffraction and powder diffraction analysis
Source: Bacillus lentus. Organism_taxid: 1467. Expressed in: bacillus licheniformis. Expression_system_taxid: 1402
Chain: A (269 residues) CATH domain: 3.40.50.200
4cfz
Savinase crystal structures for combined single crystal diffraction and powder diffraction analysis
Source: Bacillus lentus. Organism_taxid: 1467. Expressed in: bacillus licheniformis. Expression_system_taxid: 1402
Chain: A (269 residues) CATH domain: 3.40.50.200
4cg0
Savinase crystal structures for combined single crystal diffraction and powder diffraction analysis
Source: Bacillus lentus. Organism_taxid: 1467. Expressed in: bacillus licheniformis. Expression_system_taxid: 1402
Chain: A (269 residues) CATH domain: 3.40.50.200
4dww
Crystal structure of nattokinase from bacillus subtilis natt
Source: Bacillus subtilis subsp. Natto. Organism_taxid: 86029
Chain: A (275 residues) CATH domain: 3.40.50.200
5sic
Molecular recognition at the active site of subtilisin bpn': crystallographic studies using genetically engineered proteinaceous inhibitor ssi (streptomyces subtilisin inhibitor)
Source: Bacillus amyloliquefaciens. Organism_taxid: 1390.
Chain: E (275 residues) CATH domain: 3.40.50.200