PDBsum entry 1v5i

Hydrolase/protein binding

PDB id: 1v5i

Contents

Protein chains

275 a.a. *

76 a.a. *

Ligands

SO4 ×2

GOL ×16

Metals

_CA

Waters ×405

* Residue conservation analysis

PDB id:

1v5i

Name:

Hydrolase/protein binding

Title:

Crystal structure of serine protease inhibitor poia1 in complex with subtilisin bpn'

Structure:

Subtilisin bpn'. Chain: a. Engineered: yes. Mutation: yes. Ia-1=serine proteinase inhibitor. Chain: b. Synonym: poia1. Engineered: yes. Mutation: yes

Source:

Bacillus amyloliquefaciens. Organism_taxid: 1390. Expressed in: escherichia coli. Expression_system_taxid: 562. Pleurotus ostreatus. Oyster mushroom. Organism_taxid: 5322. Gene: poia1. Expression_system_taxid: 562

Biol. unit:

Dimer (from PQS)

Resolution:

1.50Å R-factor: 0.180 R-free: 0.192

Authors:

W.C.Lee,M.Kikkawa,S.Kojima,K.Miura,M.Tanokura

Key ref:

W.C.Lee et al. Crystal structure of serine protease inhibitor poia1 in complex with subtilisin bpn'. To be published, .

Date:

24-Nov-03 Release date: 08-Mar-05

Protein chain

P00782  (SUBT_BACAM) -  Subtilisin BPN' from Bacillus amyloliquefaciens

Seq: 382 a.a.

Struc: 275 a.a.*

Protein chain

Q7M4T6  (PIA1_PLEOS) -  Serine proteinase inhibitor IA-1 from Pleurotus ostreatus

Seq: 76 a.a.

Struc: 76 a.a.*

* PDB and UniProt seqs differ at 7 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chain A: E.C.3.4.21.62 - subtilisin.
• Reaction: Hydrolysis of proteins with broad specificity for peptide bonds, and a preference for a large uncharged residue in P1. Hydrolyzes peptide amides.