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UniProt functional annotation for P00782 | ||
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| UniProt code: P00782. |
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| Organism: | |
Bacillus amyloliquefaciens (Bacillus velezensis). |
| Taxonomy: | |
Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus; Bacillus amyloliquefaciens group. |
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| Function: | |
Subtilisin is an extracellular alkaline serine protease, it catalyzes the hydrolysis of proteins and peptide amides. Has a high substrate specificity to fibrin. {ECO:0000269|PubMed:12524032}. |
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| Catalytic activity: | |
Reaction=Hydrolysis of proteins with broad specificity for peptide bonds, and a preference for a large uncharged residue in P1. Hydrolyzes peptide amides.; EC=3.4.21.62; |
| Cofactor: | |
Name=Ca(2+); Xref=ChEBI:CHEBI:29108; Note=Binds 2 calcium ions per subunit.; |
| Activity regulation: | |
Completely inhibited by phenylmethylsulfony fluoride (PMSF) and partially inhibited by benzamidine hydrochloride, leupeptin, and pepstatin A. {ECO:0000269|PubMed:12524032}. |
| Biophysicochemical properties: | |
pH dependence: Optimum pH is 9.0. {ECO:0000269|PubMed:12524032}; Temperature dependence: Optimum temperature is 48 degrees Celsius. {ECO:0000269|PubMed:12524032}; |
| Subunit: | |
Monomer. {ECO:0000269|PubMed:12524032}. |
| Subcellular location: | |
Secreted. |
| Biotechnology: | |
Used as a detergent protease. Sold under the name Alcalase by Novozymes. |
| Miscellaneous: | |
Secretion of subtilisin is associated with onset of sporulation, and many mutations which block sporulation at early stages affect expression levels of subtilisin. However, subtilisin is not necessary for normal sporulation. |
| Similarity: | |
Belongs to the peptidase S8 family. {ECO:0000305}. |
| Sequence caution: | |
Sequence=CAA24990.1; Type=Erroneous initiation; Evidence={ECO:0000305}; |
Annotations taken from UniProtKB at the EBI.