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PDBsum entry 1dqo
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Sugar binding protein PDB id
1dqo

 

 

 

 

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Contents
Protein chain
134 a.a. *
Ligands
ASG
Waters ×153
* Residue conservation analysis
PDB id:
1dqo
Name: Sugar binding protein
Title: Crystal structure of the cysteine rich domain of mannose receptor complexed with acetylgalactosamine-4-sulfate
Structure: Mannose receptor. Chain: a. Fragment: cysteine rich domain. Engineered: yes
Source: Mus musculus. House mouse. Organism_taxid: 10090. Cell_line: epithelial cell. Cell: macrophage. Expressed in: homo sapiens. Expression_system_taxid: 9606.
Resolution:
2.20Å     R-factor:   0.211     R-free:   0.232
Authors: Y.Liu,A.J.Chirino,Z.Misulovin,C.Leteux,T.Feizi,M.C.Nussenzweig, P.J.Bjorkman
Key ref: Y.Liu et al. (2000). Crystal structure of the cysteine-rich domain of mannose receptor complexed with a sulfated carbohydrate ligand. J Exp Med, 191, 1105-1116. PubMed id: 10748229
Date:
04-Jan-00     Release date:   10-May-00    
PROCHECK
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 Headers
 References

Protein chain
Q61830  (MRC1_MOUSE) -  Macrophage mannose receptor 1 from Mus musculus
Seq:
Struc: 		 
Seq:
Struc: 		 
Seq:
Struc: 		1456 a.a.
134 a.a.
Key:    Secondary structure  CATH domain

 

 
J Exp Med 191:1105-1116 (2000)
PubMed id: 10748229  
 
 
Crystal structure of the cysteine-rich domain of mannose receptor complexed with a sulfated carbohydrate ligand.
Y.Liu, A.J.Chirino, Z.Misulovin, C.Leteux, T.Feizi, M.C.Nussenzweig, P.J.Bjorkman.
 
  ABSTRACT  
 
The macrophage and epithelial cell mannose receptor (MR) binds carbohydrates on foreign and host molecules. Two portions of MR recognize carbohydrates: tandemly arranged C-type lectin domains facilitate carbohydrate-dependent macrophage uptake of infectious organisms, and the NH(2)-terminal cysteine-rich domain (Cys-MR) binds to sulfated glycoproteins including pituitary hormones. To elucidate the mechanism of sulfated carbohydrate recognition, we determined crystal structures of Cys-MR alone and complexed with 4-sulfated-N-acetylgalactosamine at 1.7 and 2.2 A resolution, respectively. Cys-MR folds into an approximately three-fold symmetric beta-trefoil shape resembling fibroblast growth factor. The sulfate portions of 4-sulfated-N-acetylgalactosamine and an unidentified ligand found in the native crystals bind in a neutral pocket in the third lobe. We use the structures to rationalize the carbohydrate binding specificities of Cys-MR and compare the recognition properties of Cys-MR with other beta-trefoil proteins.
 

Literature references that cite this PDB file's key reference

  PubMed id Reference
21400496 S.K.Singh, I.Streng-Ouwehand, M.Litjens, H.Kalay, S.Burgdorf, E.Saeland, C.Kurts, W.W.Unger, and Y.van Kooyk (2011).
Design of neo-glycoconjugates that target the mannose receptor and enhance TLR-independent cross-presentation and Th1 polarization.
  Eur J Immunol, 41, 916-925.  
20106926 J.P.Upham, D.Pickett, T.Irimura, E.M.Anders, and P.C.Reading (2010).
Macrophage receptors for influenza A virus: role of the macrophage galactose-type lectin and mannose receptor in viral entry.
  J Virol, 84, 3730-3737.  
19224860 J.Lai, O.K.Bernhard, S.G.Turville, A.N.Harman, J.Wilkinson, and A.L.Cunningham (2009).
Oligomerization of the macrophage mannose receptor enhances gp120-mediated binding of HIV-1.
  J Biol Chem, 284, 11027-11038.  
19270385 K.Matsuo, H.Namatame, M.Taniguchi, and K.Gekko (2009).
Vacuum-ultraviolet circular dichroism analysis of glycosaminoglycans by synchrotron-radiation spectroscopy.
  Biosci Biotechnol Biochem, 73, 557-561.  
18798567 L.Maveyraud, H.Niwa, V.Guillet, D.I.Svergun, P.V.Konarev, R.A.Palmer, W.J.Peumans, P.Rougé, E.J.Van Damme, C.D.Reynolds, and L.Mourey (2009).
Structural basis for sugar recognition, including the Tn carcinoma antigen, by the lectin SNA-II from Sambucus nigra.
  Proteins, 75, 89.
PDB codes: 3c9z 3ca0 3ca1 3ca3 3ca4 3ca5 3ca6 3cah
18434610 F.Marttila-Ichihara, R.Turja, M.Miiluniemi, M.Karikoski, M.Maksimow, J.Niemelä, L.Martinez-Pomares, M.Salmi, and S.Jalkanen (2008).
Macrophage mannose receptor on lymphatics controls cell trafficking.
  Blood, 112, 64-72.  
  17277441 N.Kulahin, V.Kiselyov, A.Kochoyan, O.Kristensen, J.S.Kastrup, V.Berezin, E.Bock, and M.Gajhede (2007).
Structure of rat acidic fibroblast growth factor at 1.4 A resolution.
  Acta Crystallogr Sect F Struct Biol Cryst Commun, 63, 65-68.
PDB code: 2j3p
16449664 C.Oetke, M.C.Vinson, C.Jones, and P.R.Crocker (2006).
Sialoadhesin-deficient mice exhibit subtle changes in B- and T-cell populations and reduced immunoglobulin M levels.
  Mol Cell Biol, 26, 1549-1557.  
16619293 L.Martinez-Pomares, D.Wienke, R.Stillion, E.J.McKenzie, J.N.Arnold, J.Harris, E.McGreal, R.B.Sim, C.M.Isacke, and S.Gordon (2006).
Carbohydrate-independent recognition of collagens by the macrophage mannose receptor.
  Eur J Immunol, 36, 1074-1082.  
16869982 O.Reiner, F.M.Coquelle, B.Peter, T.Levy, A.Kaplan, T.Sapir, I.Orr, N.Barkai, G.Eichele, and S.Bergmann (2006).
The evolving doublecortin (DCX) superfamily.
  BMC Genomics, 7, 188.  
16056240 L.Martinez-Pomares, L.G.Hanitsch, R.Stillion, S.Keshav, and S.Gordon (2005).
Expression of mannose receptor and ligands for its cysteine-rich domain in venous sinuses of human spleen.
  Lab Invest, 85, 1238-1249.  
15009426 D.R.Gliddon, J.C.Hope, G.P.Brooke, and C.J.Howard (2004).
DEC-205 expression on migrating dendritic cells in afferent lymph.
  Immunology, 111, 262-272.  
14764901 P.R.Taylor, S.Zamze, R.J.Stillion, S.Y.Wong, S.Gordon, and L.Martinez-Pomares (2004).
Development of a specific system for targeting protein to metallophilic macrophages.
  Proc Natl Acad Sci U S A, 101, 1963-1968.  
12856000 A.Rivera-Calzada, D.Robertson, J.R.MacFadyen, J.Boskovic, C.M.Isacke, and O.Llorca (2003).
Three-dimensional interplay among the ligand-binding domains of the urokinase-plasminogen-activator-receptor-associated protein, Endo180.
  EMBO Rep, 4, 807-812.  
12615891 R.M.Steinman, D.Hawiger, and M.C.Nussenzweig (2003).
Tolerogenic dendritic cells.
  Annu Rev Immunol, 21, 685-711.  
14627732 S.R.Brych, J.Kim, T.M.Logan, and M.Blaber (2003).
Accommodation of a highly symmetric core within a symmetric protein superfold.
  Protein Sci, 12, 2704-2718.
PDB codes: 1jy0 1m16 1nzk 1p63
11602634 H.Irjala, E.L.Johansson, R.Grenman, K.Alanen, M.Salmi, and S.Jalkanen (2001).
Mannose receptor is a novel ligand for L-selectin and mediates lymphocyte binding to lymphatic endothelium.
  J Exp Med, 194, 1033-1042.  
11284160 H.J.Gabius (2001).
Glycohistochemistry: the why and how of detection and localization of endogenous lectins.
  Anat Histol Embryol, 30, 3.  
11785767 H.Kogelberg, and T.Feizi (2001).
New structural insights into lectin-type proteins of the immune system.
  Curr Opin Struct Biol, 11, 635-643.  
  11534642 L.A.Johnston, and T.P.Yoshino (2001).
Larval Schistosoma mansoni excretory-secretory glycoproteins (ESPs) bind to hemocytes of Biomphalaria glabrata (Gastropoda) via surface carbohydrate binding receptors.
  J Parasitol, 87, 786-793.  
11714927 S.R.Brych, S.I.Blaber, T.M.Logan, and M.Blaber (2001).
Structure and stability effects of mutations designed to increase the primary sequence symmetry within the core region of a beta-trefoil.
  Protein Sci, 10, 2587-2599.
PDB codes: 1jqz 1jt3 1jt4 1jt5 1jt7 1jtc
10748230 C.Leteux, W.Chai, R.W.Loveless, C.T.Yuen, L.Uhlin-Hansen, Y.Combarnous, M.Jankovic, S.C.Maric, Z.Misulovin, M.C.Nussenzweig, and T.Feizi (2000).
The cysteine-rich domain of the macrophage mannose receptor is a multispecific lectin that recognizes chondroitin sulfates A and B and sulfated oligosaccharides of blood group Lewis(a) and Lewis(x) types in addition to the sulfated N-glycans of lutropin.
  J Exp Med, 191, 1117-1126.  
10944194 D.S.Roseman, and J.U.Baenziger (2000).
Molecular basis of lutropin recognition by the mannose/GalNAc-4-SO4 receptor.
  Proc Natl Acad Sci U S A, 97, 9949-9954.  
11082189 N.Iida-Tanaka, K.Fukase, H.Utsumi, and I.Ishizuka (2000).
Conformational studies on a unique bis-sulfated glycolipid using NMR spectroscopy and molecular dynamics simulations.
  Eur J Biochem, 267, 6790-6797.  
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB codes are shown on the right.

 

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