PDBsum entry 7b0h

DNA binding protein

PDB id: 7b0h

Contents

Protein chains

713 a.a.

762 a.a.

DNA/RNA

Ligands

TTP ×2

Metals

_MN ×4

_MG ×2

PDB id:

7b0h

Name:

DNA binding protein

Title:

Tgot_6g12 ternary complex

Structure:

DNA (5'-d(p Ap Ap Cp Gp Gp Cp Ap Ap Ap Tp Gp Cp G)-3'). Chain: a, c. Engineered: yes. DNA (5'-d(p Cp Gp Cp Ap Tp T)-3'). Chain: d, g. Engineered: yes. DNA polymerase. Chain: e, f. Synonym: to pol.

Source:

Synthetic: yes. Synthetic construct. Organism_taxid: 32630. Thermococcus gorgonarius. Organism_taxid: 71997. Gene: pol, pola. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008

Resolution:

3.15Å R-factor: 0.233 R-free: 0.247

Authors:

C.Samson,P.Legrand,M.Tekpinar,J.Rozenski,M.Abramov,P.Holliger, V.Pinheiro,P.Herdewijn,M.Delarue

Date:

19-Nov-20 Release date: 13-Jan-21

Protein chain

P56689  (DPOL_THEGO) -  DNA polymerase from Thermococcus gorgonarius

Seq: 773 a.a.

Struc: 713 a.a.*

Protein chain

P56689  (DPOL_THEGO) -  DNA polymerase from Thermococcus gorgonarius

Seq: 773 a.a.

Struc: 762 a.a.*

* PDB and UniProt seqs differ at 27 residue positions (black crosses)

DNA/RNA chains

A-A-C-G-G-C-A-A-A-T-G-C-G 13 bases

A-A-C-G-G-C-A-A-A-T-G-C-G 13 bases

C-G-C-A-T-T 6 bases

C-G-C-A-T-T 6 bases

Enzyme reactions

• Enzyme class: Chains E, F: E.C.2.7.7.7 - DNA-directed Dna polymerase.
• Reaction: DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate

Molecule diagrams generated from .mol files obtained from the KEGG ftp site