PDBsum entry 7b0f

DNA binding protein

PDB id: 7b0f

Contents

Protein chain

721 a.a.

DNA/RNA

Ligands

TTP

Waters ×80

PDB id:

7b0f

Name:

DNA binding protein

Title:

Tgot_6g12 binary complex

Structure:

DNA polymerase. Chain: a. Synonym: to pol. Engineered: yes. DNA (5'-d(p Cp Gp Cp Ap Tp T)-3'). Chain: b. Engineered: yes. DNA (5'-d(p Ap Ap Cp Gp Gp Cp Tp Ap Ap Tp Gp Cp G)-3'). Chain: c.

Source:

Thermococcus gorgonarius. Organism_taxid: 71997. Gene: pol, pola. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008. Synthetic: yes. Synthetic construct. Organism_taxid: 32630. Organism_taxid: 32630

Resolution:

2.80Å R-factor: 0.232 R-free: 0.242

Authors:

C.Samson,P.Legrand,M.Tekpinar,J.Rozenski,M.Abramov,P.Holliger, V.Pinheiro,P.Herdewijn,M.Delarue

Key ref:

C.Samson et al. (2020). Structural studies of hna substrate specificity in mu of an archaeal DNA polymerase obtained by directed evolution.. Biomolecules, 10, . PubMed id: 33302546

Date:

19-Nov-20 Release date: 30-Dec-20

Protein chain

P56689  (DPOL_THEGO) -  DNA polymerase from Thermococcus gorgonarius

Seq: 773 a.a.

Struc: 721 a.a.*

* PDB and UniProt seqs differ at 11 residue positions (black crosses)

DNA/RNA chains

C-G-C-A-T-T 6 bases

A-A-C-G-G-C-T-A-A-T-G-C-G 13 bases

Enzyme reactions

• Enzyme class: E.C.2.7.7.7 - DNA-directed Dna polymerase.
• Reaction: DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate

DNA(n) + 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) (Bound ligand (Het Group name = TTP) matches with 69.23% similarity) + diphosphate

Molecule diagrams generated from .mol files obtained from the KEGG ftp site