PDBsum entry 7a6h

Transcription

PDB id: 7a6h

Contents

Protein chains

1375 a.a.

1097 a.a.

343 a.a.

124 a.a.

209 a.a.

78 a.a.

189 a.a.

148 a.a.

107 a.a.

66 a.a.

107 a.a.

46 a.a.

220 a.a.

186 a.a.

512 a.a.

146 a.a.

104 a.a.

Ligands

SF4

Metals

_MG

_ZN ×7

PDB id:

7a6h

Name:

Transcription

Title:

Cryo-em structure of human apo RNA polymerase iii

Structure:

DNA-directed RNA polymerase iii subunit rpc1. Chain: a. Synonym: RNA polymerase iii subunit c1,DNA-directed RNA polymerase iii largest subunit,DNA-directed RNA polymerase iii subunit a,RNA polymerase iii 155 kda subunit,rpc155,RNA polymerase iii subunit c160. DNA-directed RNA polymerase iii subunit rpc2. Chain: b. Synonym: RNA polymerase iii subunit c2,c128,DNA-directed RNA

Source:

Homo sapiens. Human. Organism_taxid: 9606. Organism_taxid: 9606

Authors:

M.Girbig,A.D.Misiaszek,M.K.Vorlaender,C.W.Mueller

Key ref:

M.Girbig et al. (2021). Cryo-EM structures of human RNA polymerase III in its unbound and transcribing states. Nat Struct Mol Biol, 28, 210-219. PubMed id: 33558764 DOI: 10.1038/s41594-020-00555-5

Date:

25-Aug-20 Release date: 03-Feb-21

Protein chain

O14802  (RPC1_HUMAN) -  DNA-directed RNA polymerase III subunit RPC1 from Homo sapiens

Seq: 1390 a.a.

Struc: 1375 a.a.

Protein chain

Q9NW08  (RPC2_HUMAN) -  DNA-directed RNA polymerase III subunit RPC2 from Homo sapiens

Seq: 1133 a.a.

Struc: 1097 a.a.

Protein chain

O15160  (RPAC1_HUMAN) -  DNA-directed RNA polymerases I and III subunit RPAC1 from Homo sapiens

Seq: 346 a.a.

Struc: 343 a.a.

Protein chain

O75575  (RPC9_HUMAN) -  DNA-directed RNA polymerase III subunit RPC9 from Homo sapiens

Seq: 148 a.a.

Struc: 124 a.a.

Protein chain

P19388  (RPAB1_HUMAN) -  DNA-directed RNA polymerases I, II, and III subunit RPABC1 from Homo sapiens

Seq: 210 a.a.

Struc: 209 a.a.

Protein chain

P61218  (RPAB2_HUMAN) -  DNA-directed RNA polymerases I, II, and III subunit RPABC2 from Homo sapiens

Seq: 127 a.a.

Struc: 78 a.a.

Protein chain

Q9Y535  (RPC8_HUMAN) -  DNA-directed RNA polymerase III subunit RPC8 from Homo sapiens

Seq: 204 a.a.

Struc: 189 a.a.

Protein chain

P52434  (RPAB3_HUMAN) -  DNA-directed RNA polymerases I, II, and III subunit RPABC3 from Homo sapiens

Seq: 150 a.a.

Struc: 148 a.a.

Protein chain

Q9Y2Y1  (RPC10_HUMAN) -  DNA-directed RNA polymerase III subunit RPC10 from Homo sapiens

Seq: 108 a.a.

Struc: 107 a.a.*

Protein chain

P62875  (RPAB5_HUMAN) -  DNA-directed RNA polymerases I, II, and III subunit RPABC5 from Homo sapiens

Seq: 67 a.a.

Struc: 66 a.a.

Protein chain

P0DPB6  (RPAC2_HUMAN) -  DNA-directed RNA polymerases I and III subunit RPAC2 from Homo sapiens

Seq: 133 a.a.

Struc: 107 a.a.

Protein chain

P53803  (RPAB4_HUMAN) -  DNA-directed RNA polymerases I, II, and III subunit RPABC4 from Homo sapiens

Seq: 58 a.a.

Struc: 46 a.a.

Protein chain

Q9NVU0  (RPC5_HUMAN) -  DNA-directed RNA polymerase III subunit RPC5 from Homo sapiens

Seq: 708 a.a.

Struc: 220 a.a.

Protein chain

P05423  (RPC4_HUMAN) -  DNA-directed RNA polymerase III subunit RPC4 from Homo sapiens

Seq: 398 a.a.

Struc: 186 a.a.

Protein chain

Q9BUI4  (RPC3_HUMAN) -  DNA-directed RNA polymerase III subunit RPC3 from Homo sapiens

Seq: 534 a.a.

Struc: 512 a.a.

Protein chain

Q9H1D9  (RPC6_HUMAN) -  DNA-directed RNA polymerase III subunit RPC6 from Homo sapiens

Seq: 316 a.a.

Struc: 146 a.a.

Protein chain

O15318  (RPC7_HUMAN) -  DNA-directed RNA polymerase III subunit RPC7 from Homo sapiens

Seq: 223 a.a.

Struc: 104 a.a.

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: Chains A, B: E.C.2.7.7.6 - DNA-directed Rna polymerase.
• Reaction: RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

Added reference

DOI no: 10.1038/s41594-020-00555-5

Nat Struct Mol Biol 28:210-219 (2021)

PubMed id: 33558764

Cryo-EM structures of human RNA polymerase III in its unbound and transcribing states.

M.Girbig, A.D.Misiaszek, M.K.Vorländer, A.Lafita, H.Grötsch, F.Baudin, A.Bateman, C.W.Müller.

ABSTRACT

RNA polymerase III (Pol III) synthesizes transfer RNAs and other short, essential RNAs. Human Pol III misregulation is linked to tumor transformation, neurodegenerative and developmental disorders, and increased sensitivity to viral infections. Here, we present cryo-electron microscopy structures at 2.8 to 3.3 Å resolution of transcribing and unbound human Pol III. We observe insertion of the TFIIS-like subunit RPC10 into the polymerase funnel, providing insights into how RPC10 triggers transcription termination. Our structures resolve elements absent from Saccharomyces cerevisiae Pol III such as the winged-helix domains of RPC5 and an iron-sulfur cluster, which tethers the heterotrimer subcomplex to the core. The cancer-associated RPC7α isoform binds the polymerase clamp, potentially interfering with Pol III inhibition by tumor suppressor MAF1, which may explain why overexpressed RPC7α enhances tumor transformation. Finally, the human Pol III structure allows mapping of disease-related mutations and may contribute to the development of inhibitors that selectively target Pol III for therapeutic interventions.