PDBsum entry 3v6h

Transferase/DNA

PDB id: 3v6h

Contents

Protein chains

343 a.a.

DNA/RNA

Ligands

DCP ×2

Metals

_CA ×3

_MG ×4

Waters ×210

PDB id:

3v6h

Name:

Transferase/DNA

Title:

Replication of n2,3-ethenoguanine by DNA polymerases

Structure:

DNA (5'-d( Tp Cp Ap Cp (Efg) p Gp Ap Ap Tp Cp Cp Tp Tp Cp Cp Cp Cp C)-3'). Chain: t, c. Engineered: yes. Other_details: template. DNA (5'-d( Gp G Gp Gp Gp Ap Ap Gp Gp Ap Tp Tp (Doc))-3'). Chain: p, d. Engineered: yes. Other_details: primer.

Source:

Synthetic: yes. Other_details: based on previously crystallization sequence.. Sulfolobus solfataricus p2. Organism_taxid: 273057. Strain: atcc 35092 / dsm 1617 / jcm 11322 / p2. Gene: dbh, dpo4, sso2448. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

2.30Å R-factor: 0.218 R-free: 0.267

Authors:

L.Zhao

Key ref:

L.Zhao et al. (2012). Replication of N2,3-ethenoguanine by DNA polymerases. Angew Chem Int Ed Engl, 51, 5466-5469. PubMed id: 22488769

Date:

19-Dec-11 Release date: 25-Apr-12

Protein chains

Q97W02  (DPO4_SULSO) -  DNA polymerase IV from Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)

Seq: 352 a.a.

Struc: 343 a.a.

DNA/RNA chains

C-EFG-G-A-A-T-C-C-T-T-C-C-C 13 bases

G-G-G-A-A-G-G-A-T-T-DOC 11 bases

T-C-A-C-EFG-G-A-A-T-C-C-T-T-C-C-C-C 17 bases

G-G-G-G-A-A-G-G-A-T-T-DOC 12 bases

Enzyme reactions

• Enzyme class: E.C.2.7.7.7 - DNA-directed Dna polymerase.
• Reaction: DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

Added reference

Angew Chem Int Ed Engl 51:5466-5469 (2012)

PubMed id: 22488769

Replication of N2,3-ethenoguanine by DNA polymerases.

L.Zhao, P.P.Christov, I.D.Kozekov, M.G.Pence, P.S.Pallan, C.J.Rizzo, M.Egli, F.P.Guengerich.

ABSTRACT

No abstract given.