PDBsum entry 3qqc

Transcription

PDB id

3qqc

Loading ...

Contents

			Protein chains

					381 a.a.


					82 a.a.


					58 a.a.

			Metals

					_ZN ×4

PDB id:

3qqc

Links

Name:

Transcription

Title:

Crystal structure of archaeal spt4/5 bound to the rnap clamp domain

Structure:

DNA-directed RNA polymerase subunit b, DNA-directed RNA polymerase subunit a', DNA-directed RNA polymerase subunit a''. Chain: a. Fragment: DNA-directed RNA polymerase subunit b (residues 1053-1115), DNA-directed RNA polymerase subunit a' (residues 3-318), DNA-directed RNA polymerase subunit a'' (residues 334-371). Engineered: yes. Transcription antitermination protein nusg. Chain: d.

Source:

Pyrococcus furiosus. Organism_taxid: 2261. Gene: pf1563. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: pf1990. Gene: pf0255. Expression_system_taxid: 562

Resolution:

3.30Å

R-factor:

0.195

R-free:

0.247

Authors:

F.W.Martinez-Rucobo

Key ref:

F.W.Martinez-Rucobo et al. (2011). Architecture of the RNA polymerase-Spt4/5 complex and basis of universal transcription processivity. Embo J, 30, 1302-1310. PubMed id: 21386817

Date:

15-Feb-11

Release date:

23-Mar-11

PROCHECK

	Headers

	References

Protein chain

Q8U0M3 (Q8U0M3_PYRFU) - DNA-directed RNA polymerase subunit beta from Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)

Seq: Struc:

Seq: Struc:

Seq: Struc:					1117 a.a. 381 a.a.*

Protein chain

Q8U0M4 (Q8U0M4_PYRFU) - DNA-directed RNA polymerase subunit Rpo1N from Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)

Seq: Struc:

Seq: Struc:					907 a.a. 381 a.a.*

Protein chain

Q8U0M5 (RPOA2_PYRFU) - DNA-directed RNA polymerase subunit Rpo1C from Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)

Seq: Struc:					397 a.a. 381 a.a.*

Protein chain

Q8TZK1 (SPT5_PYRFU) - Transcription elongation factor Spt5 from Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)

Seq: Struc:					152 a.a. 82 a.a.

Protein chain

Q8U440 (SPT4_PYRFU) - Transcription elongation factor Spt4 from Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)

Seq: Struc:					61 a.a. 58 a.a.

Key:

PfamA domain

Secondary structure

CATH domain

* PDB and UniProt seqs differ at 323 residue positions (black crosses)



		Enzyme reactions

Enzyme class:

Chain A: E.C.2.7.7.6 - DNA-directed Rna polymerase.

[IntEnz] [ExPASy] [KEGG] [BRENDA]

Reaction:

RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate

RNA(n)	+	ribonucleoside 5'-triphosphate	=	RNA(n+1)	+	diphosphate

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

Added reference

	Embo J 30:1302-1310 (2011)
PubMed id: 21386817

Architecture of the RNA polymerase-Spt4/5 complex and basis of universal transcription processivity.
F.W.Martinez-Rucobo, S.Sainsbury, A.C.Cheung, P.Cramer.

ABSTRACT

No abstract given.

Literature references that cite this PDB file's key reference

PubMed id

Reference

23064645

S.Larochelle, R.Amat, K.Glover-Cutter, M.Sansó, C.Zhang, J.J.Allen, K.M.Shokat, D.L.Bentley, and R.P.Fisher (2012).
Cyclin-dependent kinase control of the initiation-to-elongation switch of RNA polymerase II.

Nat Struct Mol Biol, 19, 1108-1115.

21468097

V.Svetlov, and E.Nudler (2011).
Clamping the clamp of RNA polymerase.

EMBO J, 30, 1190-1191.

The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time.