PDBsum entry 3e2f

Transferase, lyase

PDB id: 3e2f

Contents

Protein chains

410 a.a. *

Ligands

GOL-GOL

GOL ×8

Waters ×140

* Residue conservation analysis

PDB id:

3e2f

Name:

Transferase, lyase

Title:

Crystal structure of mouse kynurenine aminotransferase iii, plp-bound form

Structure:

Kynurenine-oxoglutarate transaminase 3. Chain: a, b. Synonym: kynurenine-oxoglutarate transaminase iii, kynurenine aminotransferase iii, katiii, cysteine-s-conjugate beta-lyase 2. Engineered: yes

Source:

Mus musculus. Organism_taxid: 10090. Gene: ccbl2, kat3. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108.

Resolution:

2.59Å R-factor: 0.196 R-free: 0.246

Authors:

Q.Han,R.Robinson,T.Cai,D.A.Tagle,J.Li

Key ref:

Q.Han et al. (2009). Biochemical and structural properties of mouse kynurenine aminotransferase III.. Mol cell biol, 29, 784-793. PubMed id: 29712768

Date:

05-Aug-08 Release date: 30-Dec-08

Protein chains

Q71RI9  (KAT3_MOUSE) -  Kynurenine--oxoglutarate transaminase 3 from Mus musculus

Seq: 455 a.a.

Struc: 410 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class 1: E.C.2.6.1.63 - kynurenine--glyoxylate transaminase.
• Reaction:
  1. L-kynurenine + glyoxylate = kynurenate + glycine + H2O
  2. 3-hydroxy-L-kynurenine + glyoxylate = xanthurenate + glycine + H2O

L-kynurenine (Bound ligand (Het Group name = GOL) matches with 57.14% similarity) + glyoxylate = kynurenate + glycine + H2O

3-hydroxy-L-kynurenine + glyoxylate (Bound ligand (Het Group name = GOL) matches with 57.14% similarity) = xanthurenate + glycine + H2O

• Enzyme class 2: E.C.2.6.1.7 - kynurenine--oxoglutarate transaminase.

Pathway:

• Reaction: L-kynurenine + 2-oxoglutarate = kynurenate + L-glutamate + H2O

L-kynurenine (Bound ligand (Het Group name = GOL) matches with 45.45% similarity) + 2-oxoglutarate = kynurenate + L-glutamate + H2O

• Cofactor: Pyridoxal 5'-phosphate
• Enzyme class 3: E.C.4.4.1.13 - cysteine-S-conjugate beta-lyase.
• Reaction: an S-substituted L-cysteine + H2O = a thiol + pyruvate + NH4⁺

S-substituted L-cysteine + H2O = thiol + pyruvate + NH4(+) (Bound ligand (Het Group name = GOL) matches with 71.43% similarity)

• Cofactor: Pyridoxal 5'-phosphate

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site