› 8t42

Electron Microscopy
3.6Å resolution

Model of TTLL6 MTBH1-2 bound to microtubule

Released:
DOI: 10.2210/pdb8t42/pdb
PDB entry 8t42 coloured by chain, front view.
PDB entry 8t42 coloured by chain, side view.
PDB entry 8t42 coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for α-tubulin-specific and modification state-dependent glutamylation.
Mahalingan KK, Grotjahn DA, Li Y, Lander GC, Zehr EA, Roll-Mecak A
Nat Chem Biol (2024)
PMID: 38658656
Related structures: EMD-41018

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-243986 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Tubulin alpha-1B chain Chains: A, F
Molecule details ›
Chains: A, F
Length: 451 amino acids
Theoretical weight: 50.2 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P68363 (Residues: 1-451; Coverage: 100%)
Gene name: TUBA1B
Sequence domains:
Tubulin beta chain Chains: B, E
Molecule details ›
Chains: B, E
Length: 444 amino acids
Theoretical weight: 49.72 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P07437 (Residues: 1-444; Coverage: 100%)
Gene names: OK/SW-cl.56, TUBB, TUBB5
Sequence domains:
Tubulin polyglutamylase TTLL6 Chain: N
Molecule details ›
Chain: N
Length: 503 amino acids
Theoretical weight: 59.15 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: A4Q9E8 (Residues: 1-503; Coverage: 61%)
Gene name: Ttll6
Sequence domains: Tubulin-tyrosine ligase family

Ligands and Environments

3 bound ligands:
Ligand GTP 2 x GTP
Ligand MG 4 x MG
Ligand G2P 2 x G2P
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.6Å
Relevant EMDB volumes: EMD-41018
Expression system: Escherichia coli

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