› 8r4b

Electron Microscopy
3.88Å resolution

Roco protein from C. tepidum in the GTP state bound to the activating Nanobodies NbRoco1 and NbRoco2

Released:
DOI: 10.2210/pdb8r4b/pdb
PDB entry 8r4b coloured by chain, front view.
PDB entry 8r4b coloured by chain, side view.
PDB entry 8r4b coloured by chain, top view.
Source organisms:
Primary publication:
Structural insights into the GTP-driven monomerization and activation of a bacterial LRRK2 homolog using allosteric nanobodies.
Galicia C, Guaitoli G, Fislage M, Gloeckner CJ, Versées W
Elife 13 (2024)
PMID: 38666771
Related structures: EMD-18884

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-243816 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Roc domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 1107 amino acids
Theoretical weight: 124.31 KDa
Source organism: Chlorobaculum tepidum
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8KC98 (Residues: 1-1102; Coverage: 100%)
Gene name: CT1526
Sequence domains:
NbRoco1 Chain: B
Molecule details ›
Chain: B
Length: 137 amino acids
Theoretical weight: 15.21 KDa
Source organism: Lama glama
Expression system: Escherichia coli
NbRoco2 Chain: C
Molecule details ›
Chain: C
Length: 130 amino acids
Theoretical weight: 14.18 KDa
Source organism: Lama glama
Expression system: Escherichia coli

Ligands and Environments

1 bound ligand:
Ligand GSP 1 x GSP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.88Å
Relevant EMDB volumes: EMD-18884
Expression system: Escherichia coli

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