› 8opm

Electron Microscopy
3.7Å resolution

Human Coronavirus HKU1 spike glycoprotein in complex with an alpha2,8-linked 9-O-acetylated disialoside (closed state)

Released:
DOI: 10.2210/pdb8opm/pdb
PDB entry 8opm coloured by chain, front view.
PDB entry 8opm coloured by chain, side view.
PDB entry 8opm coloured by chain, top view.
Source organisms:
Primary publication:
Sialoglycan binding triggers spike opening in a human coronavirus.
Pronker MF, Creutznacher R, Drulyte I, Hulswit RJG, Li Z, van Kuppeveld FJM, Snijder J, Lang Y, Bosch BJ, Boons GJ, Frank M, de Groot RJ, Hurdiss DL
Nature (2023)
PMID: 37794193
Related structures: EMD-17076

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-222958 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (4 distinct):
General control transcription factor GCN4; Spike glycoprotein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 1326 amino acids
Theoretical weight: 147.77 KDa
Source organisms: Expression system: Homo sapiens
UniProt:
  • Canonical: E0YJ44 (Residues: 12-1266, 1273-1273, 1277-1277, 1298-1303; Coverage: 93%)
  • Canonical: P03069 (Residues: 249-252, 254-256, 258-275; Coverage: 9%)
Gene names: AAS101, AAS3, ARG9, GCN4, S, YEL009C
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: SIA, 5N6
Carbohydrate polymer
1 bound ligand:
Ligand NAG 30 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.7Å
Relevant EMDB volumes: EMD-17076
Expression system: Homo sapiens

Quick links

Citations

1 citations in other articles

The SARS-CoV-2 furin cleavage site: natural selection or smoking gun?
Lubinski et al. (2023)