› 8k9f

Electron Microscopy
2.9Å resolution

Cryo-EM structure of the photosynthetic alternative complex III from Chloroflexus aurantiacus at 2.9 angstrom

Released:
DOI: 10.2210/pdb8k9f/pdb
PDB entry 8k9f coloured by chain, front view.
PDB entry 8k9f coloured by chain, side view.
PDB entry 8k9f coloured by chain, top view.
Source organism: Chloroflexus aurantiacus J-10-fl
Primary publication:
Cryo-EM structure of HQNO-bound Alternative Complex III from the anoxygenic phototrophic bacterium Chloroflexus aurantiacus.
Xin J, Min Z, Yu L, Yuan X, Liu A, Wu W, Zhang X, He H, Wu J, Xin Y, Blankenship RE, Tian C, Xu X
Plant Cell (2024)
PMID: 38299372
Related structures: EMD-36985

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
PDBe Complex ID:
PDB-CPX-240701 (preferred)
Entry contents:
8 distinct polypeptide molecules
Macromolecules (8 distinct):
Cytochrome c7-like domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 219 amino acids
Theoretical weight: 25.25 KDa
Source organism: Chloroflexus aurantiacus J-10-fl
UniProt:
  • Canonical: A9WEV2 (Residues: 1-219; Coverage: 100%)
Gene name: Caur_0621
Sequence domains: Cytochrome c7 and related cytochrome c
4Fe-4S ferredoxin-type domain-containing protein Chain: B
Molecule details ›
Chain: B
Length: 1029 amino acids
Theoretical weight: 113.12 KDa
Source organism: Chloroflexus aurantiacus J-10-fl
UniProt:
  • Canonical: A9WEV3 (Residues: 1-1029; Coverage: 100%)
Gene name: Caur_0622
Sequence domains: 4Fe-4S dicluster domain
Polysulphide reductase NrfD Chain: C
Molecule details ›
Chain: C
Length: 486 amino acids
Theoretical weight: 55.22 KDa
Source organism: Chloroflexus aurantiacus J-10-fl
UniProt:
  • Canonical: A9WEV4 (Residues: 1-486; Coverage: 100%)
Gene name: Caur_0623
Sequence domains: Polysulphide reductase, NrfD
Quinol:cytochrome c oxidoreductase membrane protein Chain: D
Molecule details ›
Chain: D
Length: 179 amino acids
Theoretical weight: 19.65 KDa
Source organism: Chloroflexus aurantiacus J-10-fl
UniProt:
  • Canonical: A9WEV5 (Residues: 1-179; Coverage: 100%)
Gene name: Caur_0624
Sequence domains: Alternative complex III, ActD subunit
Cytochrome c domain-containing protein Chain: E
Molecule details ›
Chain: E
Length: 205 amino acids
Theoretical weight: 23.02 KDa
Source organism: Chloroflexus aurantiacus J-10-fl
UniProt:
  • Canonical: A9WEV6 (Residues: 1-205; Coverage: 100%)
Gene name: Caur_0625
Sequence domains: Cytochrome C oxidase, cbb3-type, subunit III
Quinol:cytochrome c oxidoreductase quinone-binding subunit 2 Chain: F
Molecule details ›
Chain: F
Length: 411 amino acids
Theoretical weight: 45.72 KDa
Source organism: Chloroflexus aurantiacus J-10-fl
UniProt:
  • Canonical: A9WEV7 (Residues: 1-411; Coverage: 100%)
Gene name: Caur_0626
hypothetical protein Chain: G
Molecule details ›
Chain: G
Length: 112 amino acids
Theoretical weight: 12.49 KDa
Source organism: Chloroflexus aurantiacus J-10-fl
UniProt:
  • Canonical: A9WEV8 (Residues: 1-112; Coverage: 100%)
Gene name: Caur_0627
unknown Chain: I
Molecule details ›
Chain: I
Length: 37 amino acids
Theoretical weight: 4.32 KDa
Source organism: Chloroflexus aurantiacus J-10-fl

Ligands and Environments

7 bound ligands:
Ligand HEC 6 x HEC
Ligand SF4 3 x SF4
Ligand F3S 1 x F3S
Ligand MG 1 x MG
Ligand JLQ 1 x JLQ
Ligand JL3 1 x JL3
Ligand JM9 1 x JM9
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.9Å
Relevant EMDB volumes: EMD-36985

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