› 8h0p

Electron Microscopy
3.15Å resolution

Structure of the NMB30-NMBR and Gq complex

Released:
DOI: 10.2210/pdb8h0p/pdb
PDB entry 8h0p coloured by chain, front view.
PDB entry 8h0p coloured by chain, side view.
PDB entry 8h0p coloured by chain, top view.
Source organisms:
Primary publication:
Molecular recognition of itch-associated neuropeptides by bombesin receptors
Li C, Xu Y, Liu H, Cai H, Jiang Y, Xu HE, Yin W
Cell Res. 33 184-187 (2023)
Related structures: EMD-34413

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-239110 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Neuromedin-B Chain: L
Molecule details ›
Chain: L
Length: 10 amino acids
Theoretical weight: 1.13 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P08949 (Residues: 48-57; Coverage: 10%)
Gene name: NMB
Sequence domains: Bombesin-like peptide
Neuromedin-B receptor Chain: R
Molecule details ›
Chain: R
Length: 390 amino acids
Theoretical weight: 43.49 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P28336 (Residues: 1-390; Coverage: 100%)
Gene name: NMBR
Sequence domains: 7 transmembrane receptor (rhodopsin family)
Guanine nucleotide-binding protein G(i) subunit alpha-1; Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: A
Molecule details ›
Chain: A
Length: 361 amino acids
Theoretical weight: 41.72 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P63092 (Residues: 1-3, 27-38, 40-67, 205-251, 266-346, 348-357, 359-386, 389-394; Coverage: 55%)
  • Canonical: P63096 (Residues: 4-19, 32-32, 61-181, 229-242, 309-309, 319-319, 347-348; Coverage: 41%)
Gene names: GNAI1, GNAS, GNAS1, GSP
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 343 amino acids
Theoretical weight: 37.77 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
scFv16 Chain: E
Molecule details ›
Chain: E
Length: 247 amino acids
Theoretical weight: 26.28 KDa
Source organism: Mus musculus
Expression system: Trichoplusia ni
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.15Å
Relevant EMDB volumes: EMD-34413
Expression systems:
  • Not provided
  • Trichoplusia ni

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