› 8fux

X-ray diffraction
1.2Å resolution

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101690 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Capsule polysaccharide export protein KpsC Chains: A, B
Molecule details ›
Chains: A, B
Length: 329 amino acids
Theoretical weight: 36.48 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0H2Z2W8 (Residues: 2-323; Coverage: 48%)
Gene names: APECO1_3478, kpsC
Sequence domains: Capsule polysaccharide biosynthesis protein

Ligands and Environments

6 bound ligands:
Ligand KD3 4 x KD3
Ligand C5P 1 x C5P
Ligand PXV 2 x PXV
Ligand KDO 2 x KDO
Ligand CL 2 x CL
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P21
Unit cell:
a: 58.54Å b: 80.3Å c: 65.98Å
α: 90° β: 103.039° γ: 90°
R-values:
R R work R free
0.125 0.124 0.157
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 citations in other articles

Catalytic Mechanism of Nonglycosidic C-N Bond Formation by the Pseudoglycosyltransferase Enzyme VldE.
Tsunoda et al. (2023)

2 mentions without citation

Mechanism and linkage specificities of the dual retaining β-Kdo glycosyltransferase modules of KpsC from bacterial capsule biosynthesis.
Doyle et al. (2023)
1 more