› 8fpj

Electron Microscopy
2.74Å resolution

Co-structure of the Human Metapneunomovirus RNA-dependent RNA polymerase with MRK-1

Released:
DOI: 10.2210/pdb8fpj/pdb
PDB entry 8fpj coloured by chain, front view.
PDB entry 8fpj coloured by chain, side view.
PDB entry 8fpj coloured by chain, top view.
Source organism: human metapneumovirus
Primary publication:
Conserved allosteric inhibitory site on the respiratory syncytial virus and human metapneumovirus RNA-dependent RNA polymerases.
Kleiner VA, O Fischmann T, Howe JA, Beshore DC, Eddins MJ, Hou Y, Mayhood T, Klein D, Nahas DD, Lucas BJ, Xi H, Murray E, Ma DY, Getty K, Fearns R
Commun Biol 6 649 (2023)
PMID: 37337079
Related structures: EMD-29366

Function and Biology Details

Reactions catalysed: 
(1a) S-adenosyl-L-methionine + G(5')pppAACA-[mRNA] = S-adenosyl-L-homocysteine + G(5')pppAmACA-[mRNA]
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
(1a) (5')pppAACA-[mRNA] + (protein L)-L-histidine = diphosphate + (protein L)-L-histidyl-(5')phosphonato-AACA-[mRNA] + H(2)O
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180491 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
RNA-directed RNA polymerase L Chain: A
Molecule details ›
Chain: A
Length: 2042 amino acids
Theoretical weight: 235.23 KDa
Source organism: human metapneumovirus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q6WB93 (Residues: 1-2005; Coverage: 100%)
Gene name: L
Sequence domains:
Phosphoprotein Chains: B, C, D, E
Molecule details ›
Chains: B, C, D, E
Length: 310 amino acids
Theoretical weight: 34.81 KDa
Source organism: human metapneumovirus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q8B9Q8 (Residues: 1-294; Coverage: 100%)
Gene name: P
Sequence domains: Pneumovirus phosphoprotein

Ligands and Environments

1 bound ligand:
Ligand Y6L 1 x Y6L
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.74Å
Relevant EMDB volumes: EMD-29366
Expression system: Spodoptera frugiperda

Quick links

Citations

1 mention without citation

Conserved allosteric inhibitory site on the respiratory syncytial virus and human metapneumovirus RNA-dependent RNA polymerases.
Kleiner et al. (2023)