› 8fhu

X-ray diffraction
1.8Å resolution

Structure of Pyruvate dehydrogenase phosphatase regulatory subunit epitope presented by H2-Dd

Released:
DOI: 10.2210/pdb8fhu/pdb
PDB entry 8fhu coloured by chain, front view.
PDB entry 8fhu coloured by chain, side view.
PDB entry 8fhu coloured by chain, top view.
Source organisms:
Entry authors: Custodio JMF, Baker BM

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-219170 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
H-2 class I histocompatibility antigen, D-D alpha chain Chains: A, D
Molecule details ›
Chains: A, D
Length: 277 amino acids
Theoretical weight: 32.27 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P01900 (Residues: 26-301; Coverage: 81%)
Gene name: H2-D1
Sequence domains:
Beta-2-microglobulin Chains: B, E
Molecule details ›
Chains: B, E
Length: 99 amino acids
Theoretical weight: 11.7 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P01887 (Residues: 21-119; Coverage: 100%)
Gene name: B2m
Sequence domains: Immunoglobulin C1-set domain
Pyruvate dehydrogenase phosphatase regulatory subunit, mitochondrial Chains: C, F
Molecule details ›
Chains: C, F
Length: 9 amino acids
Theoretical weight: 940 Da
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q8NCN5 (Residues: 627-635; Coverage: 1%)
Gene names: KIAA1990, PDPR

Ligands and Environments

1 bound ligand:
Ligand K 2 x K
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P212121
Unit cell:
a: 56.415Å b: 104.564Å c: 169.993Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.208 0.249
Expression systems:
  • Escherichia coli BL21
  • Not provided

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