› 7opt

X-ray diffraction
2.02Å resolution

Function and Biology Details

Reaction catalysed: 
2 ferrocytochrome c + H(2)O(2) = 2 ferricytochrome c + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184629 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytochrome c peroxidase, mitochondrial Chains: A, B
Molecule details ›
Chains: A, B
Length: 328 amino acids
Theoretical weight: 36.55 KDa
Source organism: Trypanosoma cruzi
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8I1N3 (Residues: 1-328; Coverage: 100%)
Gene names: APX, C3747_335g5
Sequence domains: Peroxidase

Ligands and Environments


Cofactor: Ligand HEM 2 x HEM
3 bound ligands:
Ligand OXY 1 x OXY
Ligand NA 4 x NA
Ligand SO4 4 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P3121
Unit cell:
a: 71.615Å b: 71.615Å c: 253.289Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.16 0.16 0.23
Expression system: Escherichia coli

Quick links

Citations

1 mention without citation

Crystal structure of Trypanosoma cruzi heme peroxidase and characterization of its substrate specificity and compound I intermediate.
Freeman et al. (2022)