› 7oas

X-ray diffraction
1.82Å resolution

Structural basis for targeted p97 remodeling by ASPL as prerequisite for p97 trimethylation by METTL21D

Released:
DOI: 10.2210/pdb7oas/pdb
PDB entry 7oas coloured by chain, front view.
PDB entry 7oas coloured by chain, side view.
PDB entry 7oas coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Structural remodeling of AAA+ ATPase p97 by adaptor protein ASPL facilitates posttranslational methylation by METTL21D.
Petrović S, Roske Y, Rami B, Phan MHQ, Panáková D, Heinemann U
Proc Natl Acad Sci U S A 120 e2208941120 (2023)
PMID: 36656859

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123474 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
S-adenosyl-L-methionine-dependent methyltransferases superfamily protein Chain: A
Molecule details ›
Chain: A
Length: 229 amino acids
Theoretical weight: 25.14 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: F4HUD6 (Residues: 15-239; Coverage: 70%)
Gene name: At1g08125
Sequence domains: Lysine methyltransferase

Ligands and Environments

2 bound ligands:
Ligand SAH 1 x SAH
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P41212
Unit cell:
a: 58.295Å b: 58.295Å c: 142.851Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.197 0.238
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Structural insights of the p97/VCP AAA+ ATPase: How adapter interactions coordinate diverse cellular functionality.
Braxton et al. (2023)
3 other articles

1 mention without citation

Structural remodeling of AAA+ ATPase p97 by adaptor protein ASPL facilitates posttranslational methylation by METTL21D.
Petrović et al. (2023)