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Directionality of light absorption and emission in representative fluorescent proteins.

Myšková J, Rybakova O, Brynda J, Khoroshyy P, Bondar A, Lazar J
Proc Natl Acad Sci U S A 117 32395-32401 (2020)
Related entries: 6ylm, 6ylo, 6ylp, 6ylq, 6yls

Cited: 9 times
EuropePMC logo PMID: 33273123

Abstract

Fluorescent molecules are like antennas: The rate at which they absorb light depends on their orientation with respect to the incoming light wave, and the apparent intensity of their emission depends on their orientation with respect to the observer. However, the directions along which the most important fluorescent molecules in biology, fluorescent proteins (FPs), absorb and emit light are generally not known. Our optical and X-ray investigations of FP crystals have now allowed us to determine the molecular orientations of the excitation and emission transition dipole moments in the FPs mTurquoise2, eGFP, and mCherry, and the photoconvertible FP mEos4b. Our results will allow using FP directionality in studies of molecular and biological processes, but also in development of novel bioengineering and bioelectronics applications.

Articles - 6yln mentioned but not cited (1)

  1. Directionality of light absorption and emission in representative fluorescent proteins. Myšková J, Rybakova O, Brynda J, Khoroshyy P, Bondar A, Lazar J. Proc Natl Acad Sci U S A 117 32395-32401 (2020)


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  1. Local Electric Field Controls Fluorescence Quantum Yield of Red and Far-Red Fluorescent Proteins. Drobizhev M, Molina RS, Callis PR, Scott JN, Lambert GG, Salih A, Shaner NC, Hughes TE. Front Mol Biosci 8 633217 (2021)
  2. Small-Molecule Aptamer for Regulating RNA Functions in Mammalian Cells and Animals. Fukunaga K, Dhamodharan V, Miyahira N, Nomura Y, Mustafina K, Oosumi Y, Takayama K, Kanai A, Yokobayashi Y. J Am Chem Soc 145 7820-7828 (2023)
  3. AlphaFold2 and RoseTTAFold predict posttranslational modifications. Chromophore formation in GFP-like proteins. Hartley SM, Tiernan KA, Ahmetaj G, Cretu A, Zhuang Y, Zimmer M. PLoS One 17 e0267560 (2022)
  4. Defining domain-specific orientational order in the desmosomal cadherins. Dean WF, Mattheyses AL. Biophys J 121 4325-4341 (2022)
  5. Quantitative linear dichroism imaging of molecular processes in living cells made simple by open software tools. Bondar A, Rybakova O, Melcr J, Dohnálek J, Khoroshyy P, Ticháček O, Timr Š, Miclea P, Sakhi A, Marková V, Lazar J. Commun Biol 4 189 (2021)
  6. Quantitative fluorescence emission anisotropy microscopy for implementing homo-fluorescence resonance energy transfer measurements in living cells. van Zanten TS, S GP, Mayor S. Mol Biol Cell 34 tp1 (2023)


Related citations provided by authors (1)

  1. Directional optical properties of fluorescent proteins. Myskova J, Lazar J To be published -

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