› 6x3m

X-ray diffraction
3.58Å resolution

Crystal structure of full-length Streptococcal bacteriophage hyaluronidase in complex with unsaturated hyaluronan octa-saccharides

Released:
DOI: 10.2210/pdb6x3m/pdb
PDB entry 6x3m coloured by chain, front view.
PDB entry 6x3m coloured by chain, side view.
PDB entry 6x3m coloured by chain, top view.
Source organism: Streptococcus pyogenes phage H4489A
Entry authors: Deivanayagam C, Schormann N

Function and Biology Details

Reaction catalysed: 
Random hydrolysis of (1->4)-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147313 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Hyaluronoglucosaminidase Chains: A, B, C, D, E, F, G, H, I
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I
Length: 379 amino acids
Theoretical weight: 40.64 KDa
Source organism: Streptococcus pyogenes phage H4489A
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P15316 (Residues: 1-371; Coverage: 100%)
Gene names: HYLP, HYLP1
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BDP, GCD
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BDP, GCD
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: C2
Unit cell:
a: 338.168Å b: 56.797Å c: 276.954Å
α: 90° β: 126.68° γ: 90°
R-values:
R R work R free
0.25 0.247 0.299
Expression system: Escherichia coli BL21(DE3)

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