› 6w88

X-ray diffraction
3.2Å resolution

K2P2.1 (TREK-1):ML335 complex, 30 mM K+

Released:
DOI: 10.2210/pdb6w88/pdb
PDB entry 6w88 coloured by chain, front view.
PDB entry 6w88 coloured by chain, side view.
PDB entry 6w88 coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
K2P channel C-type gating involves asymmetric selectivity filter order-disorder transitions.
Lolicato M, Natale AM, Abderemane-Ali F, Crottès D, Capponi S, Duman R, Wagner A, Rosenberg JM, Grabe M, Minor DL
Sci Adv 6 (2020)
PMID: 33127683

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161303 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Potassium channel subfamily K member 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 312 amino acids
Theoretical weight: 34.3 KDa
Source organism: Mus musculus
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P97438 (Residues: 36-337; Coverage: 71%)
Gene name: Kcnk2
Sequence domains: Ion channel

Ligands and Environments

9 bound ligands:
Ligand Q6F 2 x Q6F
Ligand CD 4 x CD
Ligand D12 3 x D12
Ligand D10 2 x D10
Ligand LNK 2 x LNK
Ligand OCT 3 x OCT
Ligand R16 3 x R16
Ligand K 5 x K
Ligand UND 2 x UND
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P212121
Unit cell:
a: 67.006Å b: 119.996Å c: 128.794Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.261 0.259 0.294
Expression system: Komagataella pastoris

Quick links

Citations

5 review citations

Structural Insights into the Mechanisms and Pharmacology of K2P Potassium Channels.
Natale et al. (2021)
4 more