› 6r88

X-ray diffraction
1.6Å resolution

Structure of Arabidopsis thaliana GLR3.3 ligand-binding domain in complex with glycine

Released:
DOI: 10.2210/pdb6r88/pdb
PDB entry 6r88 coloured by chain, front view.
PDB entry 6r88 coloured by chain, side view.
PDB entry 6r88 coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
The structural bases for agonist diversity in an Arabidopsis thaliana glutamate receptor-like channel.
Alfieri A, Doccula FG, Pederzoli R, Grenzi M, Bonza MC, Luoni L, Candeo A, Romano Armada N, Barbiroli A, Valentini G, Schneider TR, Bassi A, Bolognesi M, Nardini M, Costa A
Proc Natl Acad Sci U S A 117 752-760 (2020)
PMID: 31871183

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189928 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutamate receptor 3.3 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 247 amino acids
Theoretical weight: 26.95 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9C8E7 (Residues: 463-570, 681-813; Coverage: 27%)
Gene names: At1g42540, GLR3.3, T8D8.1
Sequence domains:

Ligands and Environments

5 bound ligands:
Ligand GLY 4 x GLY
Ligand GOL 5 x GOL
Ligand NA 3 x NA
Ligand CL 2 x CL
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P212121
Unit cell:
a: 96.972Å b: 98.239Å c: 114.256Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.153 0.151 0.181
Expression system: Escherichia coli

Quick links

Citations

14 review citations

Structure, Function, and Pharmacology of Glutamate Receptor Ion Channels.
Hansen et al. (2021)
13 more

2 mentions without citation

The structural bases for agonist diversity in an Arabidopsis thaliana glutamate receptor-like channel.
Alfieri et al. (2020)
1 more