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The Plasticity of Molecular Interactions Governs Bacterial Microcompartment Shell Assembly.

Greber BJ, Sutter M, Kerfeld CA
Structure 27 749-763.e4 (2019)
Related entries: 6mzu, 6mzv, 6mzx, 6mzy, 6n07, 6n09, 6n0f, 6n0g

Cited: 22 times
EuropePMC logo PMID: 30833088

Abstract

Bacterial microcompartments (BMCs) are composed of an enzymatic core encapsulated by a selectively permeable protein shell that enhances catalytic efficiency. Many pathogenic bacteria derive competitive advantages from their BMC-based catabolism, implicating BMCs as drug targets. BMC shells are of interest for bioengineering due to their diverse and selective permeability properties and because they self-assemble. A complete understanding of shell composition and organization is a prerequisite for biotechnological applications. Here, we report the cryoelectron microscopy structure of a BMC shell at 3.0-Å resolution, using an image-processing strategy that allowed us to determine the previously uncharacterized structural details of the interactions formed by the BMC-TS and BMC-TD shell subunits in the context of the assembled shell. We found unexpected structural plasticity among these interactions, resulting in distinct shell populations assembled from varying numbers of the BMC-TS and BMC-TD subunits. We discuss the implications of these findings on shell assembly and function.

Articles - 6n06 mentioned but not cited (1)

  1. The Plasticity of Molecular Interactions Governs Bacterial Microcompartment Shell Assembly. Greber BJ, Sutter M, Kerfeld CA. Structure 27 749-763.e4 (2019)


Reviews citing this publication (7)

  1. Evolutionary relationships among shell proteins of carboxysomes and metabolosomes. Melnicki MR, Sutter M, Kerfeld CA. Curr Opin Microbiol 63 1-9 (2021)
  2. Advances in the World of Bacterial Microcompartments. Stewart AM, Stewart KL, Yeates TO, Bobik TA. Trends Biochem Sci 46 406-416 (2021)
  3. Prokaryotic Organelles: Bacterial Microcompartments in E. coli and Salmonella. Stewart KL, Stewart AM, Bobik TA. EcoSal Plus 9 (2020)
  4. Engineered bacterial microcompartments: apps for programming metabolism. Kerfeld CA, Sutter M. Curr Opin Biotechnol 65 225-232 (2020)
  5. Recent structural insights into bacterial microcompartment shells. Ochoa JM, Yeates TO. Curr Opin Microbiol 62 51-60 (2021)
  6. Selective molecular transport across the protein shells of bacterial microcompartments. Bobik TA, Stewart AM. Curr Opin Microbiol 62 76-83 (2021)
  7. Modeling bacterial microcompartment architectures for enhanced cyanobacterial carbon fixation. Trettel DS, Pacheco SL, Laskie AK, Gonzalez-Esquer CR. Front Plant Sci 15 1346759 (2024)

Articles citing this publication (14)

  1. A catalog of the diversity and ubiquity of bacterial microcompartments. Sutter M, Melnicki MR, Schulz F, Woyke T, Kerfeld CA. Nat Commun 12 3809 (2021)
  2. Structure of a Synthetic β-Carboxysome Shell. Sutter M, Laughlin TG, Sloan NB, Serwas D, Davies KM, Kerfeld CA. Plant Physiol 181 1050-1058 (2019)
  3. Incorporation of Functional Rubisco Activases into Engineered Carboxysomes to Enhance Carbon Fixation. Chen T, Fang Y, Jiang Q, Dykes GF, Lin Y, Price GD, Long BM, Liu LN. ACS Synth Biol 11 154-161 (2022)
  4. Bacterial metabolosomes: new insights into their structure and bioengineering. Liu LN. Microb Biotechnol 14 88-93 (2021)
  5. Structures of Three Actinobacteriophage Capsids: Roles of Symmetry and Accessory Proteins. Podgorski J, Calabrese J, Alexandrescu L, Jacobs-Sera D, Pope W, Hatfull G, White S. Viruses 12 E294 (2020)
  6. Rubisco forms a lattice inside alpha-carboxysomes. Metskas LA, Ortega D, Oltrogge LM, Blikstad C, Lovejoy DR, Laughlin TG, Savage DF, Jensen GJ. Nat Commun 13 4863 (2022)
  7. Engineering the PduT shell protein to modify the permeability of the 1,2-propanediol microcompartment of Salmonella. Chowdhury C, Bobik TA. Microbiology (Reading) 165 1355-1364 (2019)
  8. Functionalization of Bacterial Microcompartment Shell Proteins With Covalently Attached Heme. Huang J, Ferlez BH, Young EJ, Kerfeld CA, Kramer DM, Ducat DC. Front Bioeng Biotechnol 7 432 (2019)
  9. Intrinsically disordered CsoS2 acts as a general molecular thread for α-carboxysome shell assembly. Ni T, Jiang Q, Ng PC, Shen J, Dou H, Zhu Y, Radecke J, Dykes GF, Huang F, Liu LN, Zhang P. Nat Commun 14 5512 (2023)
  10. Linking the Salmonella enterica 1,2-Propanediol Utilization Bacterial Microcompartment Shell to the Enzymatic Core via the Shell Protein PduB. Kennedy NW, Mills CE, Abrahamson CH, Archer AG, Shirman S, Jewett MC, Mangan NM, Tullman-Ercek D. J Bacteriol 204 e0057621 (2022)
  11. Variety of size and form of GRM2 bacterial microcompartment particles. Cesle EE, Filimonenko A, Tars K, Kalnins G. Protein Sci 30 1035-1043 (2021)
  12. Single-particle cryo-EM analysis of the shell architecture and internal organization of an intact α-carboxysome. Evans SL, Al-Hazeem MMJ, Mann D, Smetacek N, Beavil AJ, Sun Y, Chen T, Dykes GF, Liu LN, Bergeron JRC. Structure 31 677-688.e4 (2023)
  13. Vertex protein PduN tunes encapsulated pathway performance by dictating bacterial metabolosome morphology. Mills CE, Waltmann C, Archer AG, Kennedy NW, Abrahamson CH, Jackson AD, Roth EW, Shirman S, Jewett MC, Mangan NM, Olvera de la Cruz M, Tullman-Ercek D. Nat Commun 13 3746 (2022)
  14. Viral Phrenology. Wilson DP, Roof DA. Viruses 13 2191 (2021)


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