› 5xc4

X-ray diffraction
1.42Å resolution

Crystal structure of GH45 endoglucanase EG27II at pH4.0, in complex with cellobiose

Released:
DOI: 10.2210/pdb5xc4/pdb
PDB entry 5xc4 coloured by chain, front view.
PDB entry 5xc4 coloured by chain, side view.
PDB entry 5xc4 coloured by chain, top view.
Source organism: Ampullaria crossean
Primary publication:
High-resolution crystal structures of the glycoside hydrolase family 45 endoglucanase EG27II from the snail Ampullaria crossean.
Nomura T, Iwase H, Saka N, Takahashi N, Mikami B, Mizutani K
Acta Crystallogr D Struct Biol 75 426-436 (2019)
PMID: 30988259

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-108276 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Endo-beta-1,4-glucanase Chain: A
Molecule details ›
Chain: A
Length: 190 amino acids
Theoretical weight: 20.92 KDa
Source organism: Ampullaria crossean
Expression system: Komagataella pastoris
UniProt:
  • Canonical: A7KMF0 (Residues: 17-195; Coverage: 100%)
Gene name: EG27II
Structure domains: RlpA-like domain

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: P212121
Unit cell:
a: 34.77Å b: 47.318Å c: 93.324Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.114 0.112 0.148
Expression system: Komagataella pastoris

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