› 5wb7

X-ray diffraction
2.94Å resolution

Crystal structure of the epidermal growth factor receptor extracellular region in complex with epiregulin

Released:
DOI: 10.2210/pdb5wb7/pdb
PDB entry 5wb7 coloured by chain, front view.
PDB entry 5wb7 coloured by chain, side view.
PDB entry 5wb7 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
EGFR Ligands Differentially Stabilize Receptor Dimers to Specify Signaling Kinetics.
Freed DM, Bessman NJ, Kiyatkin A, Salazar-Cavazos E, Byrne PO, Moore JO, Valley CC, Ferguson KM, Leahy DJ, Lidke DS, Lemmon MA
Cell 171 683-695.e18 (2017)
PMID: 28988771

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127220 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (6 distinct):
Epidermal growth factor receptor Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 507 amino acids
Theoretical weight: 56.52 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P00533 (Residues: 25-525; Coverage: 42%)
Gene names: EGFR, ERBB, ERBB1, HER1
Sequence domains:
Epiregulin Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 62 amino acids
Theoretical weight: 7.04 KDa
Source organism: Homo sapiens
Expression system: Drosophila melanogaster
UniProt:
  • Canonical: O14944 (Residues: 56-116; Coverage: 44%)
Gene name: EREG
Structure domains: Laminin

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
1 bound ligand:
Ligand NAG 7 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P21
Unit cell:
a: 76.647Å b: 199.288Å c: 87.916Å
α: 90° β: 96.74° γ: 90°
R-values:
R R work R free
0.227 0.225 0.268
Expression systems:
  • Spodoptera frugiperda
  • Drosophila melanogaster

Quick links

Citations

37 review citations

Emerging functions of the EGFR in cancer.
Sigismund et al. (2018)
36 more

18 mentions without citation

Receptor-Receptor Interactions as a Widespread Phenomenon: Novel Targets for Drug Development?
Guidolin et al. (2019)
17 more