› 5nv9

X-ray diffraction
1.95Å resolution

Substrate-bound outward-open state of a Na+-coupled sialic acid symporter reveals a novel Na+-site

Released:
DOI: 10.2210/pdb5nv9/pdb
PDB entry 5nv9 coloured by chain, front view.
PDB entry 5nv9 coloured by chain, side view.
PDB entry 5nv9 coloured by chain, top view.
Source organism: Proteus mirabilis HI4320
Primary publication:
Substrate-bound outward-open structure of a Na+-coupled sialic acid symporter reveals a new Na+ site.
Wahlgren WY, Dunevall E, North RA, Paz A, Scalise M, Bisignano P, Bengtsson-Palme J, Goyal P, Claesson E, Caing-Carlsson R, Andersson R, Beis K, Nilsson UJ, Farewell A, Pochini L, Indiveri C, Grabe M, Dobson RCJ, Abramson J, Ramaswamy S, Friemann R
Nat Commun 9 1753 (2018)
PMID: 29717135

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110080 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sodium/sialic acid symporter SiaT Chain: A
Molecule details ›
Chain: A
Length: 496 amino acids
Theoretical weight: 54.93 KDa
Source organism: Proteus mirabilis HI4320
Expression system: Escherichia coli
UniProt:
  • Canonical: B4EZY7 (Residues: 1-496; Coverage: 100%)
Gene names: PMI2976, siaT
Sequence domains: Sodium:solute symporter family

Ligands and Environments

4 bound ligands:
Ligand NA 2 x NA
Ligand SLB 1 x SLB
Ligand LMT 1 x LMT
Ligand PO4 1 x PO4
1 modified residue:
Ligand MSE 14 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: C2
Unit cell:
a: 130.59Å b: 98.07Å c: 54.78Å
α: 90° β: 92.15° γ: 90°
R-values:
R R work R free
0.201 0.199 0.243
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Heteromeric Solute Carriers: Function, Structure, Pathology and Pharmacology.
Fairweather et al. (2021)
6 more

16 mentions without citation

Changes in Membrane Protein Structural Biology.
Birch et al. (2020)
15 more