Function and Biology Details
Reactions catalysed:
GTP + a 5'-diphospho-[mRNA] = diphosphate + a 5'-(5'-triphosphoguanosine)-[mRNA]
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Biochemical function:
Biological process:
Cellular component:
- not assigned
Structure analysis Details
Assembly composition:
hetero tetramer (preferred)
Assembly name:
3C-like proteinase nsp5 and peptide (preferred)
PDBe Complex ID:
PDB-CPX-143090 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
3C-like proteinase nsp5
Molecule details ›
Chains: A, B
Length: 307 amino acids
Theoretical weight: 33.17 KDa
Source organism: Porcine epidemic diarrhea virus CV777
Expression system: Escherichia coli
UniProt:
Sequence domains: Coronavirus endopeptidase C30
Structure domains:
Length: 307 amino acids
Theoretical weight: 33.17 KDa
Source organism: Porcine epidemic diarrhea virus CV777
Expression system: Escherichia coli
UniProt:
- Canonical:
P0C6V6 (Residues: 2998-3299; Coverage: 7%)
Sequence domains: Coronavirus endopeptidase C30
Structure domains:
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
X-ray source:
PHOTON FACTORY BEAMLINE BL-5A
Spacegroup:
R3
Expression systems:
- Escherichia coli
- Not provided
