› 5eph

X-ray diffraction
1.79Å resolution

Crystal structure of extended-spectrum beta-lactamase BEL-1 in complex with Imipenem

Released:
DOI: 10.2210/pdb5eph/pdb
PDB entry 5eph coloured by chain, front view.
PDB entry 5eph coloured by chain, side view.
PDB entry 5eph coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Crystal Structure of the Pseudomonas aeruginosa BEL-1 Extended-Spectrum β-Lactamase and Its Complexes with Moxalactam and Imipenem.
Pozzi C, De Luca F, Benvenuti M, Poirel L, Nordmann P, Rossolini GM, Mangani S, Docquier JD
Antimicrob Agents Chemother 60 7189-7199 (2016)
PMID: 27671060

Function and Biology Details

Reaction catalysed: 
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174779 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-lactamase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 265 amino acids
Theoretical weight: 28.92 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q3SAW3 (Residues: 19-283; Coverage: 100%)
Gene names: bla(BEL1), blaBEL-1
Sequence domains:
Structure domains: DD-peptidase/beta-lactamase superfamily

Ligands and Environments

3 bound ligands:
Ligand CL 5 x CL
Ligand ID1 4 x ID1
Ligand MRD 1 x MRD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P21
Unit cell:
a: 53.98Å b: 95.86Å c: 103.42Å
α: 90° β: 91.97° γ: 90°
R-values:
R R work R free
0.165 0.164 0.202
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 citation in other articles

Mechanistic Insights into β-Lactamase-Catalysed Carbapenem Degradation Through Product Characterisation.
Lohans et al. (2019)
5 more

2 mentions without citation

Crystal Structure of the Pseudomonas aeruginosa BEL-1 Extended-Spectrum β-Lactamase and Its Complexes with Moxalactam and Imipenem.
Pozzi et al. (2016)
1 more