› 5ctg

X-ray diffraction
3.1Å resolution

The 3.1 A resolution structure of a eukaryotic SWEET transporter

Released:
DOI: 10.2210/pdb5ctg/pdb
PDB entry 5ctg coloured by chain, front view.
PDB entry 5ctg coloured by chain, side view.
PDB entry 5ctg coloured by chain, top view.
Source organism: Oryza sativa Japonica Group
Primary publication:
Structure of a eukaryotic SWEET transporter in a homotrimeric complex.
Tao Y, Cheung LS, Li S, Eom JS, Chen LQ, Xu Y, Perry K, Frommer WB, Feng L
Nature 527 259-263 (2015)
PMID: 26479032

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177628 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bidirectional sugar transporter SWEET2b Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 224 amino acids
Theoretical weight: 24.83 KDa
Source organism: Oryza sativa Japonica Group
Expression system: Komagataella phaffii CBS 7435
UniProt:
  • Canonical: Q5N8J1 (Residues: 1-215; Coverage: 94%)
Gene names: LOC_Os01g50460, Os01g0700100, OsJ_03146, P0047E11.3, P0454A11.22, SWEET2B
Sequence domains: Sugar efflux transporter for intercellular exchange

Ligands and Environments

3 bound ligands:
Ligand PE5 2 x PE5
Ligand BNG 1 x BNG
Ligand TRS 3 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P21
Unit cell:
a: 51.83Å b: 142.24Å c: 81.57Å
α: 90° β: 93.36° γ: 90°
R-values:
R R work R free
0.255 0.253 0.291
Expression system: Komagataella phaffii CBS 7435

Quick links

Citations

9 review citations

GLUT, SGLT, and SWEET: Structural and mechanistic investigations of the glucose transporters.
Deng et al. (2016)
8 more

16 mentions without citation

Regulation of Amino Acid Transport in Saccharomyces cerevisiae.
Bianchi et al. (2019)
15 more