› 5ayo

X-ray diffraction
3.3Å resolution

Crystal structure of a bacterial homologue of iron transporter ferroportin in inward-facing state

Released:
DOI: 10.2210/pdb5ayo/pdb
PDB entry 5ayo coloured by chain, front view.
PDB entry 5ayo coloured by chain, side view.
PDB entry 5ayo coloured by chain, top view.
Source organism: Bdellovibrio bacteriovorus HD100
Primary publication:
Outward- and inward-facing structures of a putative bacterial transition-metal transporter with homology to ferroportin.
Taniguchi R, Kato HE, Font J, Deshpande CN, Wada M, Ito K, Ishitani R, Jormakka M, Nureki O
Nat Commun 6 8545 (2015)
PMID: 26461048

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179891 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ferreportin Chain: A
Molecule details ›
Chain: A
Length: 440 amino acids
Theoretical weight: 48.6 KDa
Source organism: Bdellovibrio bacteriovorus HD100
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6MLJ0 (Residues: 1-433; Coverage: 98%)
Gene names: Bd2019, slc39
Sequence domains: Ferroportin1 (FPN1)

Ligands and Environments

3 bound ligands:
Ligand K 1 x K
Ligand ZN 2 x ZN
Ligand OLC 1 x OLC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL32XU
Spacegroup: P212121
Unit cell:
a: 63.53Å b: 70.68Å c: 101.79Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.24 0.235 0.285
Expression system: Escherichia coli

Quick links

Citations

18 review citations

A Red Carpet for Iron Metabolism.
Muckenthaler et al. (2017)
17 more

14 mentions without citation

Structure of hepcidin-bound ferroportin reveals iron homeostatic mechanisms.
Billesbølle et al. (2020)
13 more