› 5na8

X-ray diffraction
3.29Å resolution

Structure of DPP III from Bacteroides thetaiotaomicron in closed form

Released:
DOI: 10.2210/pdb5na8/pdb
PDB entry 5na8 coloured by chain, front view.
PDB entry 5na8 coloured by chain, side view.
PDB entry 5na8 coloured by chain, top view.
Source organism: Bacteroides thetaiotaomicron VPI-5482
Primary publication:
Crystal structure of dipeptidyl peptidase III from the human gut symbiont Bacteroides thetaiotaomicron.
Sabljić I, Meštrović N, Vukelić B, Macheroux P, Gruber K, Luić M, Abramić M
PLoS One 12 e0187295 (2017)
PMID: 29095893

Function and Biology Details

Reaction catalysed: 
Release of an N-terminal dipeptide from a peptide comprising four or more residues, with broad specificity. Also acts on dipeptidyl 2-naphthylamides.
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183719 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dipeptidyl-peptidase III Chains: A, B
Molecule details ›
Chains: A, B
Length: 683 amino acids
Theoretical weight: 77.69 KDa
Source organism: Bacteroides thetaiotaomicron VPI-5482
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8A6N1 (Residues: 1-675; Coverage: 100%)
Gene name: BT_1846
Sequence domains: Peptidase family M49

Ligands and Environments

2 bound ligands:
Ligand ZN 2 x ZN
Ligand SO4 4 x SO4
1 modified residue:
Ligand MSE 22 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ELETTRA BEAMLINE 5.2R
Spacegroup: P21212
Unit cell:
a: 123.632Å b: 176.315Å c: 74.177Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.207 0.251
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

The Microbiotic Highway to Health-New Perspective on Food Structure, Gut Microbiota, and Host Inflammation.
Hansen et al. (2018)
1 more

4 mentions without citation

Crystal structure of dipeptidyl peptidase III from the human gut symbiont Bacteroides thetaiotaomicron.
Sabljić et al. (2017)
3 more