› 4uou

X-ray diffraction
2.4Å resolution

Crystal Structure of Fucose binding lectin from Aspergillus Fumigatus (AFL) - apo-form

Released:
DOI: 10.2210/pdb4uou/pdb
PDB entry 4uou coloured by chain, front view.
PDB entry 4uou coloured by chain, side view.
PDB entry 4uou coloured by chain, top view.
Source organism: Aspergillus fumigatus
Primary publication:
Structural insights into Aspergillus fumigatus lectin specificity: AFL binding sites are functionally non-equivalent.
Houser J, Komarek J, Cioci G, Varrot A, Imberty A, Wimmerova M
Acta Crystallogr D Biol Crystallogr 71 442-53 (2015)
PMID: 25760594

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175845 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fucose-specific lectin Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 314 amino acids
Theoretical weight: 34.5 KDa
Source organism: Aspergillus fumigatus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q4WW81 (Residues: 2-315; Coverage: 100%)
Gene names: AFUA_5G14740, fleA
Sequence domains: Fungal fucose-specific lectin
Structure domains: Fucose-specific lectin

Ligands and Environments

1 bound ligand:
Ligand PEG 2 x PEG
1 modified residue:
Ligand CSX 4 x CSX

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P21
Unit cell:
a: 47.631Å b: 140.218Å c: 78.791Å
α: 90° β: 92.15° γ: 90°
R-values:
R R work R free
0.221 0.217 0.279
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Histo-blood group antigens as mediators of infections.
Heggelund et al. (2017)
1 more

2 mentions without citation

FICD acts bifunctionally to AMPylate and de-AMPylate the endoplasmic reticulum chaperone BiP.
Preissler et al. (2017)
1 more