› 4u8u

X-ray diffraction
3.2Å resolution

The Crystallographic structure of the giant hemoglobin from Glossoscolex paulistus at 3.2 A resolution.

Released:
DOI: 10.2210/pdb4u8u/pdb
PDB entry 4u8u coloured by chain, front view.
PDB entry 4u8u coloured by chain, side view.
PDB entry 4u8u coloured by chain, top view.
Source organism: Glossoscolex paulistus
Primary publication:
The structure of the giant haemoglobin from Glossoscolex paulistus.
Ruggiero Bachega JF, Vasconcelos Maluf F, Andi B, D'Muniz Pereira H, Falsarella Carazzollea M, Orville AM, Tabak M, Brandão-Neto J, Garratt RC, Horjales Reboredo E
Acta Crystallogr D Biol Crystallogr 71 1257-71 (2015)
PMID: 26057666

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 180-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102432 (preferred)
Entry contents:
7 distinct polypeptide molecules
Macromolecules (7 distinct):
Extracellular globin Chains: A, E, I, P, T, X, e, i, m
Molecule details ›
Chains: A, E, I, P, T, X, e, i, m
Length: 150 amino acids
Theoretical weight: 17.24 KDa
Source organism: Glossoscolex paulistus
UniProt:
  • Canonical: A0A0M3KKX5 (Residues: 1-150; Coverage: 100%)
Sequence domains: Globin
Structure domains: Globins
Extracellular globin Chains: B, F, J, Q, U, Y, f, j, n
Molecule details ›
Chains: B, F, J, Q, U, Y, f, j, n
Length: 142 amino acids
Theoretical weight: 16.25 KDa
Source organism: Glossoscolex paulistus
UniProt:
  • Canonical: A0A0M3KKX6 (Residues: 1-142; Coverage: 100%)
Sequence domains: Globin
Structure domains: Globins
Extracellular globin Chains: C, G, K, R, V, Z, g, k, o
Molecule details ›
Chains: C, G, K, R, V, Z, g, k, o
Length: 151 amino acids
Theoretical weight: 16.94 KDa
Source organism: Glossoscolex paulistus
UniProt:
  • Canonical: A0A0M3KKX7 (Residues: 1-151; Coverage: 100%)
Sequence domains: Globin
Structure domains: Globins
Extracellular globin Chains: D, H, L, S, W, a, h, l, p
Molecule details ›
Chains: D, H, L, S, W, a, h, l, p
Length: 141 amino acids
Theoretical weight: 16.12 KDa
Source organism: Glossoscolex paulistus
UniProt:
  • Canonical: A0A0M3KKX8 (Residues: 1-141; Coverage: 100%)
Sequence domains: Globin
Structure domains: Globins
Annelid erythrocruorin linker subunit C-terminal domain-containing protein Chains: M, b, q
Molecule details ›
Chains: M, b, q
Length: 224 amino acids
Theoretical weight: 25.65 KDa
Source organism: Glossoscolex paulistus
UniProt:
  • Canonical: A0A0M3KKX9 (Residues: 1-224; Coverage: 100%)
Sequence domains: Annelid erythrocruorin linker subunit C-terminus
Structure domains:
Annelid erythrocruorin linker subunit C-terminal domain-containing protein Chains: N, c, r
Molecule details ›
Chains: N, c, r
Length: 236 amino acids
Theoretical weight: 26.78 KDa
Source organism: Glossoscolex paulistus
UniProt:
  • Canonical: A0A0M3KKY0 (Residues: 1-236; Coverage: 100%)
Sequence domains:
Structure domains:
Annelid erythrocruorin linker subunit C-terminal domain-containing protein Chains: O, d, s
Molecule details ›
Chains: O, d, s
Length: 218 amino acids
Theoretical weight: 24.73 KDa
Source organism: Glossoscolex paulistus
UniProt:
  • Canonical: A0A0M3KKY1 (Residues: 1-218; Coverage: 100%)
Sequence domains:
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand HEM 36 x HEM
Ligand CYN 36 x CYN
Ligand CA 9 x CA
Ligand ZN 6 x ZN
Ligand NAG 3 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X26C
Spacegroup: I222
Unit cell:
a: 272.68Å b: 319.9Å c: 333.18Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.216 0.235

Quick links

Citations

1 review citation

Immunological properties of oxygen-transport proteins: hemoglobin, hemocyanin and hemerythrin.
Coates et al. (2017)
3 other articles

3 mentions without citation

PDBe: improved accessibility of macromolecular structure data from PDB and EMDB.
Velankar et al. (2016)
2 more