› 4mkh

X-ray diffraction
1.5Å resolution

Crystal structure of a stable adenylate kinase variant AKv18

Released:
DOI: 10.2210/pdb4mkh/pdb
PDB entry 4mkh coloured by chain, front view.
PDB entry 4mkh coloured by chain, side view.
PDB entry 4mkh coloured by chain, top view.
Source organism: Bacillus subtilis subsp. subtilis str. 168
Primary publication:
An integrated approach for thermal stabilization of a mesophilic adenylate kinase.
Moon S, Jung DK, Phillips GN, Bae E
Proteins 82 1947-59 (2014)
PMID: 24615904

Function and Biology Details

Reaction catalysed: 
ATP + AMP = 2 ADP
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147678 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Adenylate kinase Chain: A
Molecule details ›
Chain: A
Length: 222 amino acids
Theoretical weight: 24.97 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
Expression system: Escherichia coli
UniProt:
  • Canonical: P16304 (Residues: 1-212; Coverage: 98%)
Gene names: BSU01370, adk
Sequence domains:
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

3 bound ligands:
Ligand ZN 1 x ZN
Ligand AP5 1 x AP5
Ligand EDO 3 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 7A (6B, 6C1)
Spacegroup: P21
Unit cell:
a: 44.194Å b: 55.194Å c: 46.375Å
α: 90° β: 110.61° γ: 90°
R-values:
R R work R free
0.173 0.171 0.218
Expression system: Escherichia coli

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Citations

7 citation in other articles

Structural analyses of adenylate kinases from Antarctic and tropical fishes for understanding cold adaptation of enzymes.
Moon et al. (2017)
6 more